DNA polymerase: Difference between revisions

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{{STRUCTURE_1taq|  PDB=1taq  | SIZE=350| SCENE=DNA_polymerase/Cv/1 |right|CAPTION=Family A DNA polymerase complex with octylglucoside [[1taq]] }}
{{STRUCTURE_1taq|  PDB=1taq  | SIZE=350| SCENE=DNA_polymerase/Cv/1 |right|CAPTION=Family A DNA polymerase complex with octylglucoside [[1taq]] }}


[[DNA polymerase]] '''(Dpo)''' reads a [[DNA|DNA strand]] and catalyzes the synthesis of its complementary strand.  Dpo's are divided into 7 families according to their sequence homology and 3D structure similarities. The families are:<br />
[[DNA polymerase]] '''(Dpo)''' reads a [[DNA|DNA strand]] and catalyzes the synthesis of its complementary strand.  Dpo's are divided into 7 families according to their sequence homology and 3D structure similarities.<ref>PMID:10364165</ref> The families are:<br />
* Family A - replicative or repair Dpo.<br />
* Family A - replicative or repair Dpo.<br />
* Family B - replicative Dpo involved in processing DNA replication during cell division (includes eukaryotic Dpo α,δ,ε).<br />
* Family B - replicative Dpo involved in processing DNA replication during cell division (includes eukaryotic Dpo α,δ,ε).<br />
Line 363: Line 363:
**[[4i2g]] – mTdT catalytic domain + Mg + Co + DNA<br / >
**[[4i2g]] – mTdT catalytic domain + Mg + Co + DNA<br / >
}}
}}
 
== References ==
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Revision as of 17:49, 31 December 2015

Template:STRUCTURE 1taq

DNA polymerase (Dpo) reads a DNA strand and catalyzes the synthesis of its complementary strand. Dpo's are divided into 7 families according to their sequence homology and 3D structure similarities.[1] The families are:

  • Family A - replicative or repair Dpo.
  • Family B - replicative Dpo involved in processing DNA replication during cell division (includes eukaryotic Dpo α,δ,ε).
  • Family C - major replicative Dpo in bacteria (includes Dpo II, III, IV).
  • Family D - replicative. Present in archaea.
  • Family X - eukaryotic repair Dpo (includes Dpo β,λ,σ,μ and terminal deoxynucleotidyl transferase).
  • Family Y - replicative of damaged DNA (includes eukaryotic η,ι,κ).
  • Family RT - reverse transcriptase. See Reverse transcriptase.

Some Dpo terminology:
Dpo sliding clamp is made of the complex of Dpo and Proliferating Cell Nuclear Antigen (PCNA) which encircles it.
The BRCT domain in Dpo is the C-terminal domain of breast cancer susceptibility protein.
Klenow fragment is a large Dpo fragment produced upon cleavage of Dpo by subtilisin.
In the E. coli, the EcDpo III subunits β, γ, δ, δ' are named clamp loader. This complex assembles the β subunit sliding clamp unto the DNA.
Rev1 is a Dpo involved in replication over DNA lesions.
Terminal deoxynucleotidyl transferase (TdT) is a family X Dpo expressed in immature lymphoid cells. TdT adds nucleotides to exons during antibody gene recombination.
For more details see

3D Structures of DNA polymerase3D Structures of DNA polymerase

Updated on 31-December-2015

ReferencesReferences

  1. ↑ Steitz TA. DNA polymerases: structural diversity and common mechanisms. J Biol Chem. 1999 Jun 18;274(25):17395-8. PMID:10364165

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