DNA polymerase: Difference between revisions

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**[[1jih]] – yDpo η catalytic domain<br />
**[[1jih]] – yDpo η catalytic domain<br />
**[[2lsk]] - mDpo κ + DNA repair protein REV1<br />
**[[2lsk]] - mDpo κ + DNA repair protein REV1<br />
**[[2rdi]] - SsDpo IV - ''Sulfolobus solfataricus''<br />
**[[3pr4]], [[3pr5]], [[2r8g]], [[2r8h]], [[2r8i]], [[2uvr]], [[2uvu]], [[2uvv]], [[2uvw]],  - SsDpo IV (mutant) + DNA + nucleotide<br />
**[[4juz]], [[4jv0]], [[4jv1]], [[4jv2]] - SsDpo IV + DNA + nucleotide<br />
**[[2xc9]], [[3m9m]], [[3m9n]], [[3m9o]], [[2v4q]], [[2v4r]], [[2ago]], [[1ryr]], [[1rys]], [[2w9a]], [[2w9b]], [[2w9c]], [[3gii]], [[3gij]], [[3gik]], [[3gil]], [[3gim]], [[3qz7]], [[3qz8]], [[3pvx]], [[3pw0]], [[3pw2]], [[3pw4]], [[3pw5]], [[3pw7]], [[3raq]], [[3rax]], [[3rb0]], [[3rb3]], [[3rb4]], [[3rb6]], [[3rbd]], [[3rbe]], [[4hyk]], [[4fbt]], [[4fbu]], [[4g3i]] - SsDpo IV + DNA<br />
**[[2xca]], [[2xcp]], [[3khg]], [[3khh]], [[3khl]], [[3khr]], [[2w8k]], [[2w8l]], [[2rdj]], [[2v9w]], [[2va2]], [[2va3]], [[2ia6]], [[2ibk]], [[2jef]], [[2jeg]], [[2jei]], [[2jej]], [[2imw]], [[2j6s]], [[2j6t]], [[2j6u]], [[2asd]], [[2asj]], [[2asl]], [[2atl]], [[2au0]], [[2c22]], [[2c28]], [[2c2d]], [[2c2e]], [[2c2r]], [[2agp]], [[2agq]], [[2bqr]], [[2bqu]], [[2br0]], [[2bq3]], [[1s9f]], [[1s97]], [[1s0m]], [[1s0n]], [[1s0o]], [[1s10]], [[1n48]], [[1n56]], [[1jx4]], [[1jxl]], [[3t5h]], [[3t5j]], [[3t5k]], [[3t5l]], [[3v6h]], [[3v6j]], [[3v6k]], [[4gc6]], [[4gc7]] - SsDpo IV + DNA + nucleotide<br />
**[[3fds]] – SsDpo IV + PCNA B + PCNA C<br />
**[[3bq2]], [[3bq0]] – SaDpo IV + DNA – ''Sulfolobus acidocaldarius''<br />
**[[3bq1]], [[4f4w]], [[4f4x]], [[4f4y]], [[4f4z]], [[4f50]]  - SaDpo IV + DNA + nucleotide<br />
**[[4nlg]] - SaDpo IV (mutant) + DNA<br />
**[[4dez]] – Dpo IV – ''Mycobacterium smegmatis''


*Prokaryotic Pol I – DNA repair
*Prokaryotic Pol I – DNA repair

Revision as of 14:21, 26 March 2015

Template:STRUCTURE 1taq

DNA polymerase (Dpo) reads a DNA strand and catalyzes the synthesis of its complementary strand. Dpo sliding clamp is made of the complex of Dpo and Proliferating Cell Nuclear Antigen (PCNA) which encircles it. The BRCT domain in Dpo is the C-terminal domain of breast cancer susceptibility protein. The Klenow fragment is a large Dpo fragment produced upon cleavage of Dpo by subtilisin. In the E. coli, the EcDpo III subunits β, γ, δ, δ' are named clamp loader. This complex assembles the β subunit sliding clamp unto the DNA. Rev1 is a Dpo involved in replication over DNA lesions. Terminal deoxynucleotidyl transferase (TdT) is a Dpo expressed in immature lymphoid cells. TdT adds nucleotides to exons during antibody gene recombination. For more details see

3D Structures of DNA polymerase3D Structures of DNA polymerase

Updated on 26-March-2015 {{#tree:id=OrganizedByTopic|openlevels=0|

  • Family A – replicative and repair Dpo
  • Family B – 3’-5’ exonuclease activity (includes eukaryotic α,δ,ε)
  • Family C – bacterial chromosomal replicative Dpo (Pol III)
    • 2hnh, 2hqa - EcDpo III subunit α catalytic fragment
    • 4jom - EcDpo III α subunit (mutant)
    • 2hpi, 2hpm - TaDpo III α subunit
    • 3e0d - TaDpo III α subunit + DNA
    • 3f2b, 3f2c, 3f2d, 4iqj – GkDpo III α subunit + DNA + nucleotide
    • 1zd7, 1zde, 3nzm - SyDpo III α subunit – Synechocystis
    • 4gig - SyDpo III α subunit (mutant)
    • 2keq, 4kl5, 4kl6 - NpDpo III α subunit – Nostoc punctiforme
    • 4lx3 - NpDpo III α subunit + nucleic acid binding tRNA/helicase
    • 1ok7 - EcDpo III + EcDpo IV C-terminal
    • 1mmi, 2pol, 4k3s, 4pnv, 4pnw - EcDpo III subunit β
    • 2xur, 3f1v, 3pwe - EcDpo III subunit β (mutant)
    • 3d1e - EcDpo III subunit β + EcDpo II C-terminal
    • 1unn - EcDpo III subunit β + EcDpo IV little finger
    • 3d1g, 3qsb, 4k3k, 4k3l, 4mjp, 4mjq, 4mjr, 4n94, 4n95, 4n96, 4n97, 4n98, 4n99, 4n9a, 4ovf, 4ovg, 4ovh, 4pnu - EcDpo III subunit β + inhibitor
    • 3bep - EcDpo III subunit β + DNA
    • 3d1f, 4k3m, 4k3o, 4k3p, 4k3q, 4k3r, 4k74 - EcDpo III β subunit + polypeptide
    • 2avt - Dpo III β subunit – Streptococcus pyogenes
    • 2awa - Dpo III β subunit – Streptococcus pneumoniae
    • 2p1j – TmDpo III exonuclease domain – Thermotoga maritima
    • 1vpk - TmDpo III β subunit
    • 3q4j, 3q4k, 3q4l, 3d1f - EcDpo III β subunit + peptide
    • 1njf, 1njg - EcDpo III subunit γ N-terminal domains 1-2
    • 2gno - TmDpo III γ subunit
    • 1jqj, 1jql - EcDpo III subunit β, δ(mutant)
    • 1a5t - EcDpo III subunit δ’
    • 1xxh, 1jr3 - EcDpo III subunit γ, δ, δ'
    • 1xxi - EcDpo III subunit γ, δ, δ' + nucleotide
    • 2gui – EcDpo III subunit ε N-terminal
    • 4gx8, 4gx9 - EcDpo III subunit α/ε<βρ />
    • 1j53, 1j54, 2gui - EcDpo III subunit ε N-terminal exonuclease domain
    • 2ido - EcDpo III subunit ε + HOT protein
    • 3glh - EcDpo III subunit δ, δ', τ
    • 3glf - EcDpo III subunit δ, δ', τ + DNA
    • 3gli - EcDpo III subunit δ, δ', τ, ξ + DNA
    • 3glg - EcDpo III subunit δ, δ', τ mutant DNA
    • 2xy8 - EcDpo III subunit ε,θ
    • 2axd, 2ae9, 1du2 - EcDpo III subunit θ - NMR
    • 2aya - EcDpo III subunit τ
    • 1em8 - EcDpo III subunit φ, χ
    • 3sxu - EcDpo III subunit ϕ,χ + peptide
    • 1bgx – TaDpo + TP7 MAB
  • Family X – eukaryotic Dpo (β,λ,σ,μ) involved in base excision repair, non-homologous end-joining and V(D)J recombination
  • Terminal deoxynucleotidyl transferase
    • 2coe – TdT BRCT domain – NMR
    • 1jms – mTdT catalytic domain + Mg
  • Terminal deoxynucleotidyl transferase binary complex
    • 4iqt, 4iqu, 4iqv, 4iqw – mTdT catalytic domain + inhibitor
    • 4i2i – mTdT catalytic domain + Mg + Zn + adenosine-pentaphosphate
    • 4i2j – mTdT catalytic domain + CTP + Zn
    • 1kej – mTdT catalytic domain + ATP derivative + Co
    • 4i2d – mTdT catalytic domain + ATP derivative + Mg
    • 4i29 – mTdT catalytic domain + AMP derivative + Mn
    • 4i2b – mTdT catalytic domain + AMPCPP + Mg
    • 4i2c – mTdT catalytic domain + AMPCPP + Mn
    • 4i2a – mTdT catalytic domain + UMP derivative + Mg
  • Terminal deoxynucleotidyl transferase ternary complex with DNA
    • 4i2e – mTdT catalytic domain + AMPCPP + Co + DNA
    • 4i2h – mTdT catalytic domain + AMPCPP + Zn + Mg + DNA
    • 4i27 – mTdT catalytic domain + TTP derivative + TMP + Mg + DNA
    • 1kdh – mTdT catalytic domain + UMP derivative + Mg + DNA
    • 4i28, 4i2f – mTdT catalytic domain + Mg + Zn + DNA
    • 4i2g – mTdT catalytic domain + Mg + Co + DNA
  • Family Y – translesion Dpo (includes eukaryotic η,ι,κ)
  • Prokaryotic Pol I – DNA repair
  • Prokaryotic Pol II – DNA repair
    • 3o59 - PhDpo II large subunit - Pyrococcus horikoshii
    • 2kxe - PhDpo II small subunit
    • 1qqc – DeDpo II
  • Prokaryotic Pol III
    • 4tr6 - Pol III β subunit – Bacillus subtilis
    • [[3p16[[, 3rb9, 4tr7 - Pol III β subunit – Mycobacterrium tuberculosis
    • 4tr8 - PaPol III β subunit – Pseudomonas aeruginosa
    • 4tsz - PaPol III β subunit + peptide
  • Prokaryotic Pol IV

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Alexander Berchansky, David Canner, Michal Harel, Joel L. Sussman, Cori Damron, Emily Bray, Jaime Prilusky