Subtilisin

Function Subtilisin is a serine protease. A 77 amino acid propeptide is cleaved from the N-terminus of pro-Sub to create the mature active enzym. See detalis in User:Tommie Hata/Introduction to Protein Engineering-Subtilisin. Selenosubtilisin is a semisynthetic selenoenzyme produced by chemical modification of subtilisin. Savinase is a subtilisin produced from a lentil. Relevance Subtilisin is widely used commercially in detergents. Structural highlights The active site of Sub contains the catalytic triad: Ser-His-Asp. The peptide inhibitor has numerous interactions with Sub and the scissile bond is flanked by Cys-Pro-Met-Val.

spinqualitylabelsall models Subtilisin (magenta) complex with streptomycin inhibitor (wheat) and Ca+2 ions (green) (PDB entry 2sic) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D Structures of Subtilisin3D Structures of Subtilisin

Updated on 04-September-2016

{"openlevels":0}

• Native mature subtilisin
  • 2z2x – TkSub - Thermococcus kodakarensis
    
  • 1sca, 1scb, 1sbc, 3unx – BsSub - Bacillus subtilis (Subtilisin Carlsberg)
    
  • 1pxq – BsSub - NMR
    
  • 1gns, 1sup, 1st2, 1s01, 1sbt, 2sbt, 2st1 – BaSub - Bacillus amyloliquefaciens
    
  • 1ea7, 3d43, 2ixt – spehricase – Sub from Bacillus sphaericus
    
  • 2gko, 3vyv, 4dww – BsSub – Bacillus subtilis
• Mutant mature subtilisin
  • 3f49, 3co0, 2zrq, 3vhq – TkSub (mutant)
    
  • 1gnv, 1dui, 1sue, 1a2q, 1aqn, 1sua, 1au9, 1ak9, 1yja, 1yjb, 1yjc, 1sbh, 1sbi, 1sub, 1suc, 1sud, 1s02 – BaSub (mutant)
    
  • 2xrm - BcSub residues 19-321 - Bacillus clausii
    
• Unautoprocessed subtilisin (pro-Sub)
  • 2z2z, 2e1p – pro-TkSub
    
  • 2zwo, 2zwp, 4jp8, 3wiu, 3wiv – pro-TKSub (mutant)
    
  • 2wv7 – pro-BcSub
    
  • 2wwt, 2x8j - pro-BcSub (mutant)
    
  • 3whi - pro-BcSub (mutant)
    
• Mature subtilisin complexed with its propeptide
  • 3cnq – BaSub
    
  • 1spb – BaSub (mutant)
    
  • 3a3n, 3a3o, 3a3p, 2z56, 2z57, 2z58, 2z2y, 2z30 – TkSub
    
  • 3vv2 – TkSub (mutant)
    
  • 1scj – BsSub
• Subtilisin complexed with polypeptide inhibitor
  • 1y1k, 1y33, 1y34, 1y3b, 1y3c, 1y3d, 1y3f, 1y48, 1y4a, 1y4d, 1tm3, 1tm4,
  • 1tm1, 1lw6 – BaSub+ chymotripsin inhibitor 2
    
  • 1tm5, 1tm7, 1tmg, 1to1, 1to2 - BaSub+ chymotrypsin inhibitor 2 (mutant)
    
  • 3sic, 5sic, 2sic – BaSub+Streptomyces inhibitor SSI
    
  • 1yu6 – BsSub+ovomucoid
    
  • 1v5i – BaSubb+POIA1 peptide
    
  • 1r0r – BsSub+ovomucoid
    
  • 1oyv – BsSub+tomato inhibitor II
    
  • 1sbn, 1sib, 2sec, 1sni, 1cse – BsSub+Elgin C
    
  • 2sni - BaSub+Elgin C2
    
  • 1mee - BpSub+Elgin C – Bacillus pumilus
• Subtilisin complexed with inhibitor
  • 3bgo – BaSub+azide
    
  • 1c3l – BsSub+Xe
    
  • 1be8 – BsSub+cinnamoyl
    
  • 1be6 - BsSub+cinnamoyl+acetonitrile
    
  • 1bfk, 1scd – BsSub+acetonitrile
    
  • 1bfu, 1af4 – BsSub+dioxane
    
  • 1av7, 1avt, 3vsb, 1vsb – BsSub+boronic acid derivatives
    
  • 1scn – BsSub+carbamate derivative
    
  • 1bh6 – BliSub DY+benzyloxycarbonyl-ala-pro-phe-chloromethyl ketone – Bacillus licheniformis
    
  • 2wuv, 2wuw – BliSub fragment+acetonitrile
    
  • 4c3u – BliSub + Ca + Cs
    
  • 4c3v – BliSub + Ca
    
• Selenosubtilisin
  • 1ubn – BaSel-Sub
    
  • 1sel – BsSel-Sub
• Savinase
  • 3bx1, 1svn, 4cfy, 4cfz, 4cg01iav, 1gci, 1jea, 1st3 – BlSav – Bacillus lentus
    
  • 1ndu, 1q5p, 1c9j, 1c9m, 1c9n – BlSav (mutant)
    
  • 1tk2, 1ndq – BlSav+gramicidin S
    
  • 4hx2 – BlSav + Ca + Zn + sermetstatin