Subtilisin

Subtilisin is a serine protease. It is widely used commercially in detergents. A 77 amino acid propeptide is cleaved from the N-terminus of pro-Sub to create the mature active enzyme. See detalis in User:Tommie Hata/Introduction to Protein Engineering-Subtilisin.

3D Structures of Subtilisin3D Structures of Subtilisin

Updated on 24-September-2014

Native mature subtilisinNative mature subtilisin

2z2x – TkSub - Thermococcus kodakarensis
1sca, 1scb, 1sbc, 3unx – BsSub - Bacillus subtilis (Subtilisin Carlsberg)
1pxq – BsSub - NMR
1gns, 1sup, 1st2, 1s01, 1sbt, 2sbt, 2st1 – BaSub - Bacillus amyloliquefaciens
1iav, 1gci, 1jea, 1st3 – BlSub - Bacillus lentus (Subtilisin Savinase)
1ea7, 3d43, 2ixt – spehricase – Sub from Bacillus sphaericus
2gko – Sub – psychrophilic Bacillus subtilis

Mutant mature subtilisinMutant mature subtilisin

3f49, 3co0, 2zrq, 3vhq – TkSub (mutant)
1gnv, 1dui, 1sue, 1a2q, 1aqn, 1sua, 1au9, 1ak9, 1yja, 1yjb, 1yjc, 1sbh, 1sbi, 1sub, 1suc, 1sud, 1s02 – BaSub (mutant)
1ndu, 1q5p, 1c9j, 1c9m, 1c9n – BlSub (mutant)
2xrm - BcSub residues 19-321 - Bacillus clausii

Unautoprocessed subtilisin (pro-Sub)Unautoprocessed subtilisin (pro-Sub)

2z2z, 2e1p – pro-TkSub
2zwo, 2zwp, 4jp8 – pro-TKSub (mutant)
2wv7 – pro-BcSub
2wwt, 2x8j - pro-BcSub (mutant)
3whi - pro-BcSub (mutant)

Mutant unautoprocessed subtilisinMutant unautoprocessed subtilisin

2zwo, 2zwp – pro-TKSub

Mature subtilisin complexed with its propeptideMature subtilisin complexed with its propeptide

3cnq – BaSub
1spb – BaSub (mutant)
3a3n, 3a3o, 3a3p, 2z56, 2z57, 2z58, 2z2y, 2z30 – TkSub
3vv2 – TkSub (mutant)
1scj – BsSub

Subtilisin complexed with polypeptide inhibitorSubtilisin complexed with polypeptide inhibitor

1y1k, 1y33, 1y34, 1y3b, 1y3c, 1y3d, 1y3f, 1y48, 1y4a, 1y4d, 1tm3, 1tm4, 1tm1, 1lw6 – BaSub+ chymotripsin inhibitor 2
1tm5, 1tm7, 1tmg, 1to1, 1to2 - BaSub+ chymotrypsin inhibitor 2 (mutant)
3sic, 5sic, 2sic – BaSub+Streptomyces inhibitor SSI
1yu6 – BsSub+ovomucoid
1v5i – BaSubb+POIA1 peptide
1tk2, 1ndq – BlSub+gramicidin S
1r0r – BsSub+ovomucoid
1oyv – BsSub+tomato inhibitor II
1sbn, 1sib, 2sec, 1sni, 1cse – BsSub+Elgin C
2sni - BaSub+Elgin C2
1mee - BpSub+Elgin C – Bacillus pumilus

Subtilisin complexed with inhibitorSubtilisin complexed with inhibitor

3bgo – BaSub+azide
1c3l – BsSub+Xe
1be8 – BsSub+cinnamoyl
1be6 - BsSub+cinnamoyl+acetonitrile
1bfk, 1scd – BsSub+acetonitrile
1bfu, 1af4 – BsSub+dioxane
1av7, 1avt, 3vsb, 1vsb – BsSub+boronic acid derivatives
1scn – BsSub+carbamate derivative
1bh6 – Sub DY+benzyloxycarbonyl-ala-pro-phe-chloromethyl ketone – Bacillus licheniformis
2wuv, 2wuw – BlSub fragment+acetonitrile

SelenosubtilisinSelenosubtilisin

1ubn – BaSel-Sub
1sel – BsSel-Sub

SavinaseSavinase

3bx1, 1svn, 4cfy, 4cfz, 4cg0 – Sav – Bacillus lentus