Thioesterase

Thioesterase (TE) catalyzes the break of an ester bond to produce acid and alcohol at a thiol group. TEs are substrate-specific. Palmitoyl protein TE removes fatty acids like palmitate from modified cysteine residues during lysosomal degradation. 4-hydroxybenzoyl-CoA TE converts 4-hydroxybenzoyl-CoA to 4-hydroxybenzoate and CoA. Acyl-CoA TE hydrolyzes acyl-CoA to the fatty acid and CoA and is involved in lipid metabolism. Fluoroacetyl-CoA TE from Streptomyces cattleya hydrolyzes fluoroacetyl-CoA thus preventing it from being metabolized to the lethal 4-hydroxy-trans-aconitate. Ubiquitin TE (USP) removes conjugated ubiquitin from proteins thus regulating protein level by preventing their degradation.

3D structures of thioesterase3D structures of thioesterase

Updated on 12-August-2013

Maristoyl-ACP-specific TE

1tht – TE – Vibrio harveyi

4-hydroxybenzoyl-CoA TE

1bvq – PsTE – Pseudomonas
1lo7 – PsTE + 4-hydroxyphenyl CoA
1lo9 - PsTE (mutant) + 4-hydroxybenzoyl CoA
1lo8 - PsTE + 4-hydroxybenzyl CoA
1q4s - ArTE + 4-hydroxybenzoate – 'Arthrobacter'
1q4t - ArTE + 4-hydroxyphenyl CoA
1q4u - ArTE + 4-hydroxybenzyl CoA
3r32, 3r35, 3r37, 3r38, 3r3c, 3r3d, 3r3f, 3tea - ArTE (mutant) + 4-hydroxyphenacyl CoA
3r3a - ArTE (mutant) + 4-hydroxybenzoate
3r34 - ArTE (mutant) + CoA

Palmitoyl protein TE

1eh5 – bTE1 + Palmitate – bovine
1ei9 – bTE1
1exw – bTE1 + hexadecylsulfonyl fluoride
1pja – hTEII – human
3gro - hTEI

Acyl-CoA TE

1c8u – EcTEII – Escherichia coli
1ivn – EcTEI
1jrl – EcTEI (mutant)
1j00 – EcTEI + diethyl phosphono derivative
1v2g, 1u8u - EcTEI + octanoic acid
2v1o – mTE7 hotdog domain – mouse
2q2b – mTE7 C terminal
3hlk – hTE2
3k2i – hTE4
2qq2 - hTE7 C terminal
3fo5 – hTE11 START domain
3b7k – hTE12
3rd7 – TE – Mycobacterium avium
3u0a – TEII – Mycobacterium marinum
1tbu – TE N terminal (peroximal) – yeast

Acyl protein TE

1fj2 – EcTEI

Acyl-ACP TE

2ess – TE – Bacterioides thetaiotaomicron

Fluoroacetyl-CoA TE

3kuv, 3kuw, 3kvi – ScTE (mutant) + acetate derivative – Streptomyces cattleya
3kv7, 3kv8, 3p2r, 3p2s – ScTE + acetate derivative
3kvu – ScTE + acetyl-CoA
3kvz, 3kw1 – ScTE + FAcOPan
3kx7, 3fx8, 3p2q – ScTE
3p3f – ScTE (mutant)
3p3i - ScTE (mutant) + acetate derivative + CoA

Ubiquitin TE

USP 2

2zfy – hUSP – human
2hd5, 3nhe – hUSP + Ub
1tff – hUSP (mutant)
2ibi – hUSP (mutant) + Ub

USP 3

2qiy – USP + UBP-associated protein – yeast

USP 5

3ihp – hUSP + Ub

USP 7

2ylm, 2f1z – hUSP
2kvr – hUSP UBL domain - NMR
1nb8 – hUSP catalytic domain
1yze – hUSP N terminal domain
2f1w – hUSP TRAF-like domain
2f1x – hUSP TRAF-like domain + p53 peptide
2foj, 2foo – hUSP N terminal domain + p53 peptide
2f1y – hUSP TRAF-like domain + MDM2 peptide
2fop – hUSP N terminal domain + MDM2 peptide
1nbf – hUSP catalytic domain + ubiquitin aldehyde
1yy6 – hUSP N terminal domain + Epstein-Barr nuclear antigen
3mqr, 3mqs – hUSP + peptide
2xxn – hUSP + VIRF-4
4jjq – hUSP (mutant) + E2

USP 8

2a9u – hUSP N terminal domain
2gfo – hUSP catalytic domain
2gwf – hUSP rhodanese domain + ring finger protein
3mhh, 3m99, 4fip, 4fjc, 4fk5 – hUSP + SUS1 + SAGA-associated factors 11, 73
3mhs – hUSP + SUS1 + SAGA-associated factors 11, 73 + Ub
3n3k – hUSP + Ub inhibitor

USP 15

3lmn – hUSP DUSP domain

USP 16

2i50 – hUSP zinc finger domain - NMR

USP 21

3i3t – hUSP + Ub
3mtn – hUSP + Ub inhibitor

USP 28

2lva – hUSP N terminal - NMR

USP 33

2uzg – hUSP zinc finger domain - NMR

USP 37

3ihr – hUSP (mutant)
3a7s – hUSP catalytic domain (mutant)

USP Cyld

2vhf – hUSP

USP L1

3irt – hUSP (mutant)
2len – hUSP (mutant) - NMR
3ifw, 3kvf, 3kw5 – hUSP (mutant) + Ub
4dm9 – hUSP + peptide inhibitor

USP L5

3ris – hUSP catalytic domain
3rii – hUSP catalytic domain (mutant)
3tb3 – hUSP UCH domain (mutant)

USP ZranB1

3zrh – hUSP

USP OTUB1

3von – hUSP + E2
4ddg, 4ddi – hUSP + Ub
4ddi – hUSP (mutant) + Ub
4dhz – hUSP + E2 + Ub
4dhj – nUSP + E2 + Ub - nematode
4dhi – nUSP + E2

3znh – USP + Ub – Crimean-Congo hemorrhagic fever virus

RedJ TE

3qmv, 3qmw – TE – Streptomyces coelicolor

2av9 – TE – Pseudomonas aeruginosa