Caspase: Difference between revisions

Revision as of 09:55, 23 June 2013

Template:STRUCTURE 1pyo

Caspase (CASP) are cysteine-aspartic proteases which function in apoptosis, necrosis and inflammation. Twelve CASP have been identified in human. CASP is synthesized as an inactive pro-CASP with a prodomain which is being cleaved off to render them active. The X-linked inhibitor of apoptosis protein (XIAP) with its baculoviral IAP repeat (BIR) domain is an inhibitor of CASP. CASP-1 cleaves precursor cytokine interleukin 1-β and interleukin 18 into mature protein. CASP-3 interacts with CASP-8 and CASP-9 during cell apoptosis. CASP-7 is a heterodimer consisting of P20 (human residues 1-198) and P11 (human residues 199-303) subunits. CASP-7 catalytic domain consists of residues 57-303. is an important initiator CASP and drICE is an effector of apoptosis CASP in Drosophila melanogaster. Metacaspase (MCASP) are arginine/lysine specific CASP. MCASP are found in plants and fungi.

For some details see:
CASP-1 - Human Caspase-1
CASP-3 - Sandox Bay Serrano; Caspase-3 Regulatory Mechanisms
CASP-6 - Molecular Playground/Caspase-6 (new); Caspase-6 and neurodegeneration
CASP-7 - Molecular Playground/Caspase-7 Dynamics
CASP-9 - Molecular Playground/Caspase-9 Regulation.

3D structures of caspase3D structures of caspase

Updated on 23-June-2013

CASP-1CASP-1

3e4c – hpro-CASP (mutant) - human
2nn3 – pro-SfCASP (mutant) – Spodoptera frugiperda
1sc1, 1sc4 - hCASP (mutant)
1sc3 - hCASP (mutant) + malonate
3d6f, 3d6h, 3d6m, 1ibc - hCASP (mutant) + polypeptide inhibitor
2hbq, 1bmq, 3ns7 - hCASP + inhibitor
2h4w, 2h4y, 2h51, 2h54, 2hbr, 2hby, 2hbz, 2h48, 2fqq, 1rwk, 1rwm, 1rwn, 1rwo, 1rwp, 1rwv, 1rww, 1rwx - hCASP (mutant) + inhibitor
1ice – hCASP + tetrapeptide aldehyde
1m72 – SfCASP + polypeptide inhibitor

CASP-2CASP-2

2p2c – hCASP
3r7s - hCASP subunits P12, P18
1pyo, 3r5j, 3r6g, 3r6l, 3r7b, 3r7n, 3rjm - hCASP + polypeptide inhibitor

CASP-3CASP-3

1qx3 - hCASP
2xyg, 2xyh, 2xyp – hCASP subunits P12, P17 + inhibitor
2xzd, 2xzt, 2y0b - hCASP subunits P12, P17 + DARPIN-3.4
3pcx, 3pd1, 3pd0, 3gjq, 3gjr, 3gjs, 3gjt, 3edq, 3edr, 2cnk, 2cnl, 2cnn, 2cno, 2cdr, 2h65, 1cp3, 1pau – hCASP + polypeptide inhibitor
4dcj, 4dco, 4dcp, 4eha, 4ehd, 4ehf, 4ehh, 4ehk, 4ehl, 4ehn – hCASP subunits P12, P17 + polypeptide inhibitor
2dko, 2cjx, 2cjy - hCASP (mutant) + polypeptide inhibitor
3kjf, 3h0e, 3deh, 3dei, 3dej, 3dek, 1re1, 1rhj, 1rhk, 1rhm, 1rhq, 1rhr, 1rhu, 1nme, 1nmq, 1nms, 1gfw – hCASP + inhibitor
3itn, 2j30, 2j31, 2j32, 2j33, 2c1e, 2c2k, 2c2m, 2c2o, 2c2z, 2h5i, 2h5j – hpro-CASP + polypeptide inhibitor
1i3o - hCASP (mutant) + XIAP-BIR2

CASP-6CASP-6

3p45, 3k7e, 2wdp – hCASP
3nkf, 3nr2, 3s8e, 3v6l, 3v6m – hCASP (mutant)
3od5, 3p4u, 3qnw, 3s70 – hCASP + polypeptide inhibitor
4ejf – hCASP subunit P11 + P18 + polypeptide
4fxo - hCASP subunit P11 + P18 + Zn

CASP-7CASP-7

3rk5, 4fdi - hCASP
3ibf – hCASP P20 subunit
3r5k - hCASP (mutant)
1k86, 1gqf – hCASP catalytic domain
1k88 – hpro-CASP (mutant)
3ibc, 2ql5, 2ql7, 2ql9, 2qlb, 2qlf, 2qlj, 3edr - hCASP P20 subunit + polypeptide inhibitor
1f1j - hCASP + polypeptide inhibitor
3h1p, 4jr1, 4jr2 - hCASP catalytic domain (mutant) + polypeptide inhibitor
1shj, 1shl - hCASP catalytic domain (mutant) + inhibitor
4fea - hCASP catalytic domain + inhibitor
1i51, 1kmc - hCASP (mutant) + XIAP-BIR2
1i4o - hCASP P20 subunit + XIAP

CASP-8CASP-8

3kjn, 3kjq – hCASP + inhibitor
1f9e, 1qtn, 1qdu - hCASP + polypeptide inhibitor
3h11 - hCASP (mutant) + polypeptide inhibitor
2k7z – hpro-CASP (mutant) – NMR
2fun, 1i4e – CASP + P35 – Autographa californica

CASP-9CASP-9

2ar9 – hCASP (mutant)
1jxq - hCASP + polypeptide inhibitor
1nw9 – hCASP catalytic domain + XIAP-BIR3
3ygs – hpro-CASP + APAF-1 CARD

Drosophila melanogaster CASPDrosophila melanogaster CASP

2fp3 – DmCASP Dronc residues 136-450 – Drosophila melanogaster
3sir - DmCASP drICE residues 78-332
3sip – DmCASP drICE residues 78-230 + apoptosis inhibitor

MetacaspaseMetacaspase

4afp, 4afr, 4afv – MCASP (mutant) – Trypanosom brucei

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

@@ Line 1: / Line 1: @@
   {{STRUCTURE_1pyo| PDB=1pyo  | SIZE=400| SCENE= |right|   CAPTION=CASP-2 subunit P18 (grey, pink) and subunit P12 (green, yellow) complex with polypeptide inhibitor (magenta, cyan), aspartic aldehyde and acetyl group, [[1pyo]] }}
-'''Caspase''' (CASP) are cysteine-aspartic proteases which function in apoptosis, necrosis and inflammation.  Twelve CASP have been identified in human.  CASP is synthesized as an inactive pro-CASP with a prodomain which is being cleaved off to render them active.  The X-linked inhibitor of apoptosis protein (XIAP) with its baculoviral IAP repeat (BIR) domain is an inhibitor of CASP.  CASP-1 cleaves precursor cytokine interleukin 1-β and interleukin 18 into mature protein.  CASP-3 interacts with CASP-8 and CASP-9 during cell apoptosis.
+'''Caspase''' (CASP) are cysteine-aspartic proteases which function in apoptosis, necrosis and inflammation.  Twelve CASP have been identified in human.  CASP is synthesized as an inactive pro-CASP with a prodomain which is being cleaved off to render them active.  The X-linked inhibitor of apoptosis protein (XIAP) with its baculoviral IAP repeat (BIR) domain is an inhibitor of CASP.  CASP-1 cleaves precursor cytokine interleukin 1-β and interleukin 18 into mature protein.  CASP-3 interacts with CASP-8 and CASP-9 during cell apoptosis.  CASP-7 is a heterodimer consisting of P20 (human residues 1-198) and P11 (human residues 199-303) subunits.  CASP-7 catalytic domain consists of residues 57-303. is an important initiator CASP and drICE is an effector of apoptosis CASP in ''Drosophila melanogaster''.  Metacaspase (MCASP) are arginine/lysine specific CASP.  MCASP are found in plants and fungi.
 For some details see:<br />
@@ Line 9: / Line 9: @@
 CASP-7 - [[Molecular Playground/Caspase-7 Dynamics]]<br />
 CASP-9 - [[Molecular Playground/Caspase-9 Regulation]].
-Dronc is an important initiator CASP and drICE is an effector of apoptosis CASP in ''Drosophila melanogaster''.  Metacaspase (MCASP) are arginine/lysine specific CASP.  MCASP are found in plants and fungi.
 __NOTOC__
@@ Line 26: / Line 26: @@
 [[2hbq]], [[1bmq]], [[3ns7]] - hCASP + inhibitor<br />
 [[2h4w]], [[2h4y]], [[2h51]], [[2h54]], [[2hbr]], [[2hby]], [[2hbz]], [[2h48]], [[2fqq]], [[1rwk]], [[1rwm]], [[1rwn]], [[1rwo]], [[1rwp]], [[1rwv]], [[1rww]], [[1rwx]] - hCASP (mutant) + inhibitor<br />
+[[1ice]] – hCASP + tetrapeptide aldehyde<br />
 [[1m72]] – SfCASP + polypeptide inhibitor<br />
@@ Line 56: / Line 57: @@
 ===CASP-7===
-[[3rk5]], [[3ibf]] – hCASP<br />
+[[3rk5]], [[4fdi]] - hCASP<br />
-[[1k86]], [[3r5k]] - hCASP (mutant) <br />
+[[3ibf]] – hCASP P20 subunit<br />
+[[3r5k]] - hCASP (mutant) <br />
+[[1k86]], [[1gqf]] – hCASP catalytic domain<br />
 [[1k88]] – hpro-CASP (mutant) <br />
-[[3ibc]], [[2ql5]], [[2ql7]], [[2ql9]], [[2qlb]], [[2qlf]], [[2qlj]], [[1f1j]] - hCASP + polypeptide inhibitor<br />
+[[3ibc]], [[2ql5]], [[2ql7]], [[2ql9]], [[2qlb]], [[2qlf]], [[2qlj]], [[3edr]] - hCASP P20 subunit + polypeptide inhibitor<br />
-[[3h1p]] - hCASP (mutant) + polypeptide inhibitor<br />
+[[1f1j]] - hCASP + polypeptide inhibitor<br />
-[[1shj]], [[1shl]] - hCASP (mutant) + inhibitor<br />
+[[3h1p]], [[4jr1]], [[4jr2]] - hCASP catalytic domain (mutant) + polypeptide inhibitor<br />
+[[1shj]], [[1shl]] - hCASP catalytic domain (mutant) + inhibitor<br />
+[[4fea]] - hCASP catalytic domain + inhibitor<br />
 [[1i51]], [[1kmc]] - hCASP (mutant) + XIAP-BIR2<br />
-[[1i4o]] - hCASP + XIAP
+[[1i4o]] - hCASP P20 subunit + XIAP
 ===CASP-8===