Subtilisin

File:2z2x.png

Crystal Structure of Subtilisin 2z2x

Subtilisin is a serine protease. It is widely used commercially in detergents. A 77 amino acid propeptide is cleaved from the N-terminus of pro-Sub to create the mature active enzyme. The images at the left and at the right correspond to one representative Subtilisin, i.e. the crystal structure of native mature subtilisin from Thermococcus kodakarensis (2z2x). See detalis in User:Tommie Hata/Introduction to Protein Engineering-Subtilisin.

3D Structures of Subtilisin3D Structures of Subtilisin

Updated December 2011

Native mature subtilisinNative mature subtilisin

2z2x – TkSub - Thermococcus kodakarensis
1sca, 1scb, 1sbc – BsSub - Bacillus subtilis (Subtilisin Carlsberg)
1pxq – BsSub - NMR
1gns, 1sup, 1st2, 1s01, 1sbt, 2sbt, 2st1 – BaSub - Bacillus amyloliquefaciens
1iav, 1gci, 1jea, 1st3 – BlSub - Bacillus lentus (Subtilisin Savinase)
1ea7, 3d43, 2ixt – spehricase – Sub from Bacillus sphaericus
2gko – Sub – psychrophilic Bacillus subtilis

Mutant mature subtilisinMutant mature subtilisin

3f49, 3co0, 2zrq – TkSubb (mutant)
1gnv, 1dui, 1sue, 1a2q, 1aqn, 1sua, 1au9, 1ak9, 1yja, 1yjb, 1yjc, 1sbh, 1sbi, 1sub, 1suc, 1sud, 1s02 – BaSub (mutant)
1ndu, 1q5p, 1c9j, 1c9m, 1c9n – BlSub (mutant)
2xrm - BcSub residues 19-321 - Bacillus clausii

Unautoprocessed subtilisin (pro-Sub)Unautoprocessed subtilisin (pro-Sub)

2z2z, 2e1p – pro-TkSub
2zwo, 2zwp – pro-TKSub (mutant)
2wv7 – pro-BcSub
2wwt, 2x8j - pro-BcSub (mutant)

Mutant unautoprocessed subtilisinMutant unautoprocessed subtilisin

2zwo, 2zwp – pro-TKSub

Mature subtilisin complexed with its propeptideMature subtilisin complexed with its propeptide

3cnq – BaSub
1spb – BaSub (mutant)
3a3n, 3a3o, 3a3p, 2z56, 2z57, 2z58, 2z2y, 2z30 – TkSub
1scj – BsSub

Subtilisin complexed with polypeptide inhibitorSubtilisin complexed with polypeptide inhibitor

1y1k, 1y33, 1y34, 1y3b, 1y3c, 1y3d, 1y3f, 1y48, 1y4a, 1y4d, 1tm3, 1tm4, 1tm1, 1lw6 – BaSub+ chymotripsin inhibitor 2
1tm5, 1tm7, 1tmg, 1to1, 1to2 - BaSub+ chymotrypsin inhibitor 2 (mutant)
3sic, 5sic, 2sic – BaSub+Streptomyces inhibitor SSI
1yu6 – BsSub+ovomucoid
1v5i – BaSubb+POIA1 peptide
1tk2, 1ndq – BlSub+gramicidin S
1r0r – BsSub+ovomucoid
1oyv – BsSub+tomato inhibitor II
1sbn, 1sib, 2sec, 1sni, 1sce – BsSub+Elgin C
2sni - BaSub+Elgin C2
1mee - BpSub+Elgin C – Bacillus pumilus

Subtilisin complexed with inhibitorSubtilisin complexed with inhibitor

3bgo – BaSub+azide
1c3l – BsSub+Xe
1be8 – BsSub+cinnamoyl
1be6 - BsSub+cinnamoyl+acetonitrile
1bfk, 1scd – BsSub+acetonitrile
1bfu, 1af4 – BsSub+dioxane
1av7, 1avt, 3vsb, 1vsb – BsSub+boronic acid derivatives
1scn – BsSub+carbamate derivative
1bh6 – Sub DY+benzyloxycarbonyl-ala-pro-phe-chloromethyl ketone – Bacillus licheniformis
2wuv, 2wuw – BlSub fragment+acetonitrile

SelenosubtilisinSelenosubtilisin

1ubn – BaSel-Sub
1sel – BsSel-Sub

SavinaseSavinase

3bx1 – Sav – Bacillus lentus