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This Sandbox is Reserved from 05/22/2012, through 07/22/2012 for use in the course "BIOL 414" taught by Greg Buhrman at the North Carolina State University, Raleigh, NC USA. This reservation includes Sandbox Reserved 551 through Sandbox Reserved 590.

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Hi Justin, Here is your very own Proteopedia Page for pdb code: 2WDL. Your presentation is scheduled for: June 11.

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Greg Buhrman

spinqualitylabelsall models PDB ID 2wdl Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
2wdl, resolution 3.50Å ()
Related:	2wdk, 2wdm, 2wdn, 2jl8, 2b66, 2j03, 1v8q, 2v47, 2j01, 2jl6, 1gd8, 1wki, 2b9p, 1yl3, 2b9n, 2v49, 1giy
Structural annotation: Resources: InterPro : Ipr001684, Ipr018261, Ipr001383, Ipr018254, Ipr001854, Ipr016082, Ipr018038, Ipr000517, Ipr005996, Ipr002150, Ipr002677, Ipr018264, Ipr001705, Ipr000271, Ipr018265, Ipr001706, Ipr000473, Ipr000923, Ipr002143, Ipr005878, Ipr016094, Ipr010916, Ipr016027, Ipr014722, Ipr012340, Ipr008991, Ipr002171, Ipr005880, Ipr000597, Ipr009000, Ipr019926, Ipr019927, Ipr015498, Ipr013005, Ipr002136, Ipr002132, Ipr000702, Ipr002358, Ipr020040, Ipr019906, Ipr020594, Ipr000244, Ipr009027, Ipr020069, Ipr020070, Ipr005823, Ipr005822, Ipr019972, Ipr005745, Ipr000218, Ipr001196, Ipr005749, Ipr000114, Ipr016180, Ipr000456, Ipr005484, Ipr004389, Ipr018257, Ipr001857, Ipr005813, Ipr001787, Ipr018258, Ipr001063, Ipr018260, Ipr005727, Ipr012678, Ipr012677, Ipr001014, Ipr013025, Ipr014723, Ipr005825, Ipr005824, Ipr003256, Ipr020056, Ipr001021, Ipr011035, Ipr020055, Ipr020057 Pfam : PF01016, PF00831, PF00327, PF01197, PF01783, PF00471, PF00468, PF01632, PF00444, PF00687, PF03947, PF00181, PF00297, PF00573, PF00673, PF00281, PF00347, PF03948, PF01281, PF00572, PF00238, PF00256, PF01305, PF00252, PF01196, PF00861, PF01245, PF00453, PF00829, PF00237, PF00276, PF00467 UniProt : P60493, P60494, Q5SHP6, Q5SHQ6, Q5SJE1, P80339, P35871, P80340, Q5SKU1, Q5SHR2, Q5SLP7, P60405, Q5SHN8, Q5SHN9, Q5SHQ0, Q5SHQ3, Q5SLQ1, P60488, Q5SHP8, Q5SHQ7, P60489, Q9Z9H5, Q5SHQ4, P60490, P60491, P60492, Q5SHP3, Q5SHP0, Q5SHP9, Q5SHZ1
Evolutionary conservation: Toggle Conservation Colors: Rows = identical sequences: Further Information: Caveats, Explanation, Complete Results at ConSurfDB
Resources:	FirstGlance, OCA, RCSB, PDBsum
Coordinates:	save as pdb, mmCIF, xml

STRUCTURE OF THE THERMUS THERMOPHILUS 70S RIBOSOME IN COMPLEX WITH MRNA, PAROMOMYCIN, ACYLATED A- AND P-SITE TRNAS, AND E-SITE TRNA. THIS FILE CONTAINS THE 50S SUBUNIT FOR MOLECULE I.STRUCTURE OF THE THERMUS THERMOPHILUS 70S RIBOSOME IN COMPLEX WITH MRNA, PAROMOMYCIN, ACYLATED A- AND P-SITE TRNAS, AND E-SITE TRNA. THIS FILE CONTAINS THE 50S SUBUNIT FOR MOLECULE I.

Publication Abstract from PubMed

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Protein synthesis is catalyzed in the peptidyl transferase center (PTC), located in the large (50S) subunit of the ribosome. No high-resolution structure of the intact ribosome has contained a complete active site including both A- and P-site tRNAs. In addition, although past structures of the 50S subunit have found no ordered proteins at the PTC, biochemical evidence suggests that specific proteins are capable of interacting with the 3' ends of tRNA ligands. Here we present structures, at 3.6-A and 3.5-A resolution respectively, of the 70S ribosome in complex with A- and P-site tRNAs that mimic pre- and post-peptidyl-transfer states. These structures demonstrate that the PTC is very similar between the 50S subunit and the intact ribosome. They also reveal interactions between the ribosomal proteins L16 and L27 and the tRNA substrates, helping to elucidate the role of these proteins in peptidyl transfer.

Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome., Voorhees RM, Weixlbaumer A, Loakes D, Kelley AC, Ramakrishnan V, Nat Struct Mol Biol. 2009 May;16(5):528-33. Epub 2009 Apr 12. PMID:19363482

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this StructureAbout this Structure

2wdl is a 31 chain structure of Ribosomal protein L1, Ribosomal protein L2, Ribosomal protein L3, Ribosomal protein L4, Ribosomal protein L5, Ribosomal protein L6 and Ribosome with sequence from Thermus thermophilus. The January 2010 RCSB PDB Molecule of the Month feature on 70S Ribosomes by David Goodsell is 10.2210/rcsb_pdb/mom_2010_1. Full crystallographic information is available from OCA.

See AlsoSee Also

• Ribosomal protein L1
• Ribosomal protein L2
• Ribosomal protein L3
• Ribosomal protein L4
• Ribosomal protein L5
• Ribosomal protein L6
• Ribosome