Luciferase

File:3iep.png Crystal Structure of Luciferase 3iep Luciferase (Luc) is an oxidative enzyme used in bioluminescence. It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light. The images at the left and at the right correspond to one representative Luc, i.e. the crystal structure of firefly Luciferase (3iep). See detailed analysis of Luciola cruciata luciferase and Luciferase FMN complex- Vibrio harveyi. 3D Structures of Luciferase Update February 2013 3iep, 1lci – fLuc – firefly 3ier – fLuc+PEG 400 1ba3 – fLuc+bromoform 3ies, 3rix, 4e5d – fLuc+inhibitor 4g36 – fLUc + dehydroluciferyl-sulfamoyl adenosine 4g37 – fLUc (mutant) + dehydroluciferyl-sulfamoyl adenosine 2d1q – LcLuc (mutant)+MgATP – Luciola cruciata 2d1r - LcLuc (mutant)+oxyluciferin+AMP 2d1s, 2d1t - LcLuc (mutant)+intermediate analog 3qya – Luc (mutant) – Lampyris turkestanicus 3fgc – VhLuc α+β chain+FMN – Vibrio harveyi 1luc, 1brl - VhLuc α+β chain 1bsl, 1xkj - VhLuc β chain 2psd, 2psh, 2psj, 2pse, 2psf – Luc (mutant) – Renilla reniformis Additional information (2d1s) Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms. Luciola cruciata luciferase (Japanese firefly), complexed with Oxyluciferin & AMP (2d1r) or with an high-energy analogue (2d1s) 3ies,3iep and 3ier Firefly luciferase (FLuc) bound to its natural substrate ATP and the inhibitor PTC124 1ba3 firefly luciferase enzyme from Photinus pyralis 2psd, 2pse, 2psf, 2psh, 2psj, and 2rh7 the luciferase from Renilla reniformis (RLuc) 1bsl 1.95Å heterodimer of bacterial luciferase from Vibrio harveyi 1brl 2.4Å heterodimer of bacterial luciferase from Vibrio harveyi 1xkj homodimer of bacterial luciferase from Vibrio harveyi 2d1q, 2d1r and 2d1s thermostable Japanese Firefly (Luciola cruciata) Luciferase complexed with High-energy intermediate analog 2d1t thermostable Japanese Firefly Luciferase red-color emission S286N mutant 1lci firefly luciferase enzyme from Photinus pyralis Dinoflagellate luciferase 1luc The 1.5Å resolution crystal structure of bacterial luciferase from Vibrio harveyi 3fgc Bacterial luciferase from Vibrio harveyi 1vpr luciferase of Lingulodinium polyedrum, a marine bioluminescent dinoflagellate Green Fluorescent Protein For additional information, see: Colored & Bioluminescent Proteins

spinqualitylabelsall models Luciferase (PDB code 3iep) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

External ResourcesExternal Resources

• Yahoo news coverage of luciferase studies funded research for military applications
• Luciferase at Wikipedia
• Luciferase: June 2006 Molecule of the Month as part of the series of tutorials that are at the RCSB Protein Data Bank and written by David S. Goodsell