Proliferating Cell Nuclear Antigen

Function

Proliferating Cell Nuclear Antigen (PCNA) acts as a DNA sliding clamp by encircling the DNA and linking it to the genome. It is a processivity factor for DNA polymerase (Dpo) δ in eukaryotic cells^[1].

Relevance

PCNA is useful in the diagnosis of high-grade dysplasia^[2].

Structural highlights

p15 regulates DNA replication and repair by binding to PCNA. PCNA-p15 peptide complex shows the peptide passes through the PCNA ring and has numerous interactions with it^[3].

Human proliferating cell nuclear antigen trimer (grey, green, pink) complex with p15 peptide (yellow, magenta) (PDB entry 4d2g)

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3D Structures of Proliferating Cell Nuclear Antigen3D Structures of Proliferating Cell Nuclear Antigen

Updated on 12-July-2016

ReferencesReferences

↑ Maga G, Hubscher U. Proliferating cell nuclear antigen (PCNA): a dancer with many partners. J Cell Sci. 2003 Aug 1;116(Pt 15):3051-60. PMID:12829735 doi:http://dx.doi.org/10.1242/jcs.00653
↑ Kullmann F, Fadaie M, Gross V, Knuchel R, Bocker T, Steinbach P, Scholmerich J, Ruschoff J. Expression of proliferating cell nuclear antigen (PCNA) and Ki-67 in dysplasia in inflammatory bowel disease. Eur J Gastroenterol Hepatol. 1996 Apr;8(4):371-9. PMID:8781908
↑ De Biasio A, de Opakua AI, Mortuza GB, Molina R, Cordeiro TN, Castillo F, Villate M, Merino N, Delgado S, Gil-Carton D, Luque I, Diercks T, Bernado P, Montoya G, Blanco FJ. Structure of p15(PAF)-PCNA complex and implications for clamp sliding during DNA replication and repair. Nat Commun. 2015 Mar 12;6:6439. doi: 10.1038/ncomms7439. PMID:25762514 doi:http://dx.doi.org/10.1038/ncomms7439

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Alexander Berchansky, Michal Harel, Joel L. Sussman, Lukáš Cakl

[1] Maga G, Hubscher U. Proliferating cell nuclear antigen (PCNA): a dancer with many partners. J Cell Sci. 2003 Aug 1;116(Pt 15):3051-60. PMID:12829735 doi:http://dx.doi.org/10.1242/jcs.00653

[2] Kullmann F, Fadaie M, Gross V, Knuchel R, Bocker T, Steinbach P, Scholmerich J, Ruschoff J. Expression of proliferating cell nuclear antigen (PCNA) and Ki-67 in dysplasia in inflammatory bowel disease. Eur J Gastroenterol Hepatol. 1996 Apr;8(4):371-9. PMID:8781908

[3] De Biasio A, de Opakua AI, Mortuza GB, Molina R, Cordeiro TN, Castillo F, Villate M, Merino N, Delgado S, Gil-Carton D, Luque I, Diercks T, Bernado P, Montoya G, Blanco FJ. Structure of p15(PAF)-PCNA complex and implications for clamp sliding during DNA replication and repair. Nat Commun. 2015 Mar 12;6:6439. doi: 10.1038/ncomms7439. PMID:25762514 doi:http://dx.doi.org/10.1038/ncomms7439

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