3p91
Crystal structure of Proliferating Cellular Nuclear Antigen from Entamoeba histolyticaCrystal structure of Proliferating Cellular Nuclear Antigen from Entamoeba histolytica
Structural highlights
FunctionC4M9R9_ENTH1 This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.[RuleBase:RU000641] Publication Abstract from PubMedProliferating cellular nuclear antigen (PCNA) is a toroidal-shaped protein that is involved in cell-cycle control, DNA replication and DNA repair. Parasitic protozoa are early-diverged eukaryotes that are responsible for neglected diseases. In this work, a PCNA from a parasitic protozoon was identified, cloned and biochemically characterized and its crystal structure was determined. Structural and biochemical studies demonstrate that PCNA from Entamoeba histolytica assembles as a homotrimer that is able to interact with and stimulate the activity of a PCNA-interacting peptide-motif protein from E. histolytica, EhDNAligI. The data indicate a conservation of the biochemical mechanisms of PCNA-mediated interactions between metazoa, yeast and parasitic protozoa. Structure and biochemical characterization of proliferating cellular nuclear antigen from a parasitic protozoon.,Cardona-Felix CS, Lara-Gonzalez S, Brieba LG Acta Crystallogr D Biol Crystallogr. 2011 Jun;67(Pt 6):497-505. Epub 2011 May 12. PMID:21636889[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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