3pvp

Structure of Mycobacterium tuberculosis DnaA-DBD in complex with box2 DNAStructure of Mycobacterium tuberculosis DnaA-DBD in complex with box2 DNA

Structural highlights 3pvp is a 6 chain structure with sequence from Mycta. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 3pvv Gene: dnaA, MRA_0001, Rv0001 (MYCTA) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [DNAA_MYCTA] Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.[HAMAP-Rule:MF_00377] Publication Abstract from PubMed An essential protein, DnaA, binds to 9-bp DNA sites within the origin of replication oriC. These binding events are prerequisite to forming an enigmatic nucleoprotein scaffold that initiates replication. The number, sequences, positions, and orientations of these short DNA sites, or DnaA boxes, within the oriCs of different bacteria vary considerably. To investigate features of DnaA boxes that are important for binding Mycobacterium tuberculosis DnaA (MtDnaA), we have determined the crystal structures of the DNA binding domain (DBD) of MtDnaA bound to a cognate MtDnaA-box (at 2.0 A resolution) and to a consensus Escherichia coli DnaA-box (at 2.3 A). These structures, complemented by calorimetric equilibrium binding studies of MtDnaA DBD in a series of DnaA-box variants, reveal the main determinants of DNA recognition and establish the [T/C][T/A][G/A]TCCACA sequence as a high-affinity MtDnaA-box. Bioinformatic and calorimetric analyses indicate that DnaA-box sequences in mycobacterial oriCs generally differ from the optimal binding sequence. This sequence variation occurs commonly at the first 2 bp, making an in vivo mycobacterial DnaA-box effectively a 7-mer and not a 9-mer. We demonstrate that the decrease in the affinity of these MtDnaA-box variants for MtDnaA DBD relative to that of the highest-affinity box TTGTCCACA is less than 10-fold. The understanding of DnaA-box recognition by MtDnaA and E. coli DnaA enables one to map DnaA-box sequences in the genomes of M. tuberculosis and other eubacteria. Structural and Thermodynamic Signatures of DNA Recognition by Mycobacterium tuberculosis DnaA.,Tsodikov OV, Biswas T J Mol Biol. 2011 May 18. PMID:21620858 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also DnaA

spinqualitylabelsall models 3pvp, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image