Vinculin

Function Vinculins (VCLs) are involved in adhesion by linking integrin molecules to the actin cytoskeleton. Its head domain (Vd1) can bind to talin or to alpha-actinin at their respective VCL Binding Sites (VBS). The protein raver1 RNA Recognition Motif (RRM) forms a complex with VCL or m-VCL. Metavinculin (m-VCL) is a splice version of VCL containing an extra ca. 70 amino acids in the C-terminal domain. Relevance Loss of VCL could be used as a prognostic factor for colorectal cancer se it promotes metastasis. Disease Mutation in m-VCL can yield cardiomyopathic phenotype. Structural highlights is achieved through a high affinity intramolecular interaction between tail (orange) and head (aqua) domains (1st6). Energetically, I997 is key to maintaining this autoinhibition.

spinqualitylabelsall models Chicken full-length metavinculin, 1st6 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D Structures of Vinculin3D Structures of Vinculin

Updated on 23-June-2022

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• Vinculin; Domains - head 140-327; tail 878-1066
  • 1tr2, 6fuy – hVCL - human
    
  • 3h2u – hVCL head domain + raver1 RRM
    
  • 2ibf, 2hsq, 2gww - hVCL head domain + SfVCL binding sites from Shigella flexneri
    
  • 1ydi - hVCL head domain +hActinin VBS
    
  • 1syq, 1rkc, 1rke – hVCL head domain +hTalin VBS
    
  • 1qkr – hVCL tail domain
    
  • 3h2v - hVCL tail domain + raver1 RRM
    
  • 4pr9 - hVCL tail domain + lipid
    
  • 3jbi - hVCL tail domain + actin – Cryo-EM
    
  • 6nr7 - cVCL – chicken
    
  • 1t01 - cVCL head domain +mTalin VBS
    
  • 3zdl – cVCL head domain + amyloid β precursor protein N terminal
    
• Metavinculin; Domains - head 1-256; tail 959-1134
  • 7ktt, 7ktu, 7ktv, 7ktw – hVCL – Cryo-EM
    
  • 6upw – hVCL + actin + ADP – Cryo-EM
    
  • 6fuy - hVCL
    
  • 3rf3 - hVCL head domain + invasin IPAA
    
  • 3s90 - hVCL head domain + mTalin-1 peptide
    
  • 4dj9 - hVCL head domain + hTalin-1 peptide
    
  • 6fq4 - hVCL head domain + TARP peptide
    
  • 3tj5 - hVCL head domain + Sca-family protein peptide
    
  • 3tj6 - hVCL head domain + protein Ps 120 peptide
    
  • 4ehp, 5y04 - hVCL head domain + catenin α-1 residues 277-382
    
  • 3myi – hVCL tail domain
    
  • 5l0f, 5l0i, 5l0j - hVCL tail domain (mutant)
    
  • 5l0c, 5l0d - hVCL tail domain + lipid
    
  • 5l0g, 5l0h - hVCL tail domain (mutant) + lipid
    
  • 3jbk – hVCL tail domain + actin – Cryo-EM
    
  • 6upw - mVCL head domain + catenin α-1
    
  • 3vf0 – hVCL tail domain + ribonucleoprotein PTB-binding
    
  • 1st6 – cVCL
    
  • 2gdc – cVCL head domain +SfInvasin C-terminal
    
  • 1xwj - cVCL head domain +cTalin VBS3
    
  • 1zvz, 1zw2, 1zw3, 1u6h - cVCL head domain +cTalin rod
    
  • 6fq4 – cVCL head domain + TARP-VBS1
    
  • 4e17, 4e18 - cVCL head domain + catenin α-1 VCL-binding domain

ReferencesReferences

• Created with the participation of Susan Craig.