Proliferating Cell Nuclear Antigen: Difference between revisions

Revision as of 16:13, 14 August 2019

Function

Proliferating Cell Nuclear Antigen (PCNA) acts as a DNA sliding clamp by encircling the DNA and linking it to the genome. It is a processivity factor for DNA polymerase (Dpo) δ in eukaryotic cells^[1].

Relevance

PCNA is useful in the diagnosis of high-grade dysplasia^[2].

Structural highlights

p15 regulates DNA replication and repair by binding to PCNA. PCNA-p15 peptide complex shows the and has ^[3].

Human proliferating cell nuclear antigen trimer (cyan, green, deeppink) complex with p15 peptide (yellow, magenta) (PDB entry 4d2g)

Drag the structure with the mouse to rotate

3D Structures of Proliferating Cell Nuclear Antigen3D Structures of Proliferating Cell Nuclear Antigen

Updated on 14-August-2019

Unliganded PCNA
- 1rwz – AfPCNA - Archaeglobus fulgidus
- 3lx1 - TkPCNA1 - Thermococcus kodakarensis
- 5a6d - PCNA - Thermococcus gammatolerans
- 3lx2 - TkPCNA2
- 3lox, 1plq, 1plr, 4yhr, 5t9d – yPCNA - yeast
- 3f1x, 3gpm, 3gpn, 3f1w, 4l60, 4l6p, 6cx2, 6cx3, 6cx4, 5v7m, 5v7l, 5v7k - yPCNA (mutant)
- 3l0x - yPCNA N fragment + C fragment
- 3pge - yPCNA N fragment + SUMO-C fragment
- 1vym, 1w60, 3vkx – hPCNA - human
- 5mom, 5e0t – hPCNA (mutant)
- 5e0t, 5mom - hPCNA (mutant)
- 2io4 – SsPCNA B (mutant) + PCNA C - Sulfolobus solfataricus
- 2hii, 2hik, 2ixi, 2ix2 - SsPCNA A + PCNA B + PCNA C
- 2nti - SsPCNA A + PCNA B (mutant) + PCNA C
- 2ijx - SsPCNA A
- 1iz4, 1iz5, 1ge8, 5auj – PfPCNA (mutant) - Pyrococcus furiosus
- 3hi8, 3ifv - PCNA - Haloferax volcanii
- 3aix, 3aiz - StPCNA B + PCNA C – Sulfolobus tokodaii
- 1ud9 - StPCNA
- 3p91 – PCNA – Entamoeba histoytiica
- 4hk1 – PCNA – Drosophila melanogaster
- 4cs5 – PCNA – Litopenaeus vannamei
- 5cfk, 5b7o, 5h0t, 5hac - PCNA - Leishmania donovani
PCNA + Dpo
- 2zvk – hPCNA + hDpo ε
- 2zvl – hPCNA + hDpo κ
- 2zvm – hPCNA + hDpo τ
- 3fds – SsPCNA B + PCNA C + SsDpo IV
- 3a2f - PfPCNA + Dpo
PCNA complexes
- 1vyj, 1u76, 1u7b, 6cbi, 5yd8, 5yco, 5mlw, 5mlo, 5mav, 5iy4, 4ztd, 4rjf – hPCNA + peptide
- 5e0u - hPCNA (mutant) + peptide
- 4d2g, 6gws – hPCNA + p15 peptide
- 6e49 – hPCNA + DNA helicase peptide
- 6eht – hPCNA + p15 peptide + DNA
- 5e0v - hPCNA + Flap structure-specific endonuclease peptide
- 4ztd - hPCNA + ubiquitin ligase peptide
- 3wgw – hPCNA + inhibitor
- 6gis – hPCNA + DNA
- 4rjf - hPCNA + p21 peptide
- 5e0u - hPCNA (mutant) + p21
- 1axc - hPCNA + P21/WAF1 C-terminat
- 1ul1 - hPCNA + Flap exonuclease-1
- 5e0v - hPCNA (mutant) + Flap endonoclease 1
- 3p87 - hPCNA + RNase H2 subunit B (mutant)
- 3tbl - hPCNA + ubiquitin
- 5iy4 - hPCNA + DVC1 PIP box
- 5mlo, 5mlw - hPCNA + ZRANB3 peptide
- 5mav - hPCNA + PARG peptidee
- 6gis - hPCNA + DNA
- 3p83 - AfPCNA + RNase H2
- 1rxm – AfPCNA + Flap exonuclease-1 peptide
- 1rxz - AfPCNA + Flap structure-specific endonuclease
- 3l0w, 3l10 - yPCNA + monoubiquinated PCNA
- 1sxj – yPCNA + replication factor C
- 2od8 - yPCNA + DNA ligase fragment
- 3k4x - yPCNA + DNA
- 3v60, 3v61 – yPCNA (mutant) + ubiquitin-like protein SMT3
- 3v62 – yPCNA (mutant) + ubiquitin-like protein SMT3 + ATP-dependent DNA helicase SRS2
- 5zut – yPCNA + peptide
- 5jne - yPCNA (mutant) + SMT3 + E2
- 2izo - SsPCNA B + PCNA C + Flap structure-specific endonuclease
- 1isq - PfPCNA (mutant) + replication factor C large subunit
- 2zvw, 2zvv - PCNA + cyclin-dependent kinase inhibitor 1 - Arabidopsis thaliana
- 5da7 - TkPCNA + protein inhibitor

ReferencesReferences

↑ Maga G, Hubscher U. Proliferating cell nuclear antigen (PCNA): a dancer with many partners. J Cell Sci. 2003 Aug 1;116(Pt 15):3051-60. PMID:12829735 doi:http://dx.doi.org/10.1242/jcs.00653
↑ Kullmann F, Fadaie M, Gross V, Knuchel R, Bocker T, Steinbach P, Scholmerich J, Ruschoff J. Expression of proliferating cell nuclear antigen (PCNA) and Ki-67 in dysplasia in inflammatory bowel disease. Eur J Gastroenterol Hepatol. 1996 Apr;8(4):371-9. PMID:8781908
↑ De Biasio A, de Opakua AI, Mortuza GB, Molina R, Cordeiro TN, Castillo F, Villate M, Merino N, Delgado S, Gil-Carton D, Luque I, Diercks T, Bernado P, Montoya G, Blanco FJ. Structure of p15(PAF)-PCNA complex and implications for clamp sliding during DNA replication and repair. Nat Commun. 2015 Mar 12;6:6439. doi: 10.1038/ncomms7439. PMID:25762514 doi:http://dx.doi.org/10.1038/ncomms7439

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Alexander Berchansky, Michal Harel, Joel L. Sussman, Lukáš Cakl

[1] Maga G, Hubscher U. Proliferating cell nuclear antigen (PCNA): a dancer with many partners. J Cell Sci. 2003 Aug 1;116(Pt 15):3051-60. PMID:12829735 doi:http://dx.doi.org/10.1242/jcs.00653

[2] Kullmann F, Fadaie M, Gross V, Knuchel R, Bocker T, Steinbach P, Scholmerich J, Ruschoff J. Expression of proliferating cell nuclear antigen (PCNA) and Ki-67 in dysplasia in inflammatory bowel disease. Eur J Gastroenterol Hepatol. 1996 Apr;8(4):371-9. PMID:8781908

[3] De Biasio A, de Opakua AI, Mortuza GB, Molina R, Cordeiro TN, Castillo F, Villate M, Merino N, Delgado S, Gil-Carton D, Luque I, Diercks T, Bernado P, Montoya G, Blanco FJ. Structure of p15(PAF)-PCNA complex and implications for clamp sliding during DNA replication and repair. Nat Commun. 2015 Mar 12;6:6439. doi: 10.1038/ncomms7439. PMID:25762514 doi:http://dx.doi.org/10.1038/ncomms7439

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@@ Line 7: / Line 7: @@
 == Structural highlights ==
-p15 regulates DNA replication and repair by binding to PCNA.  PCNA-p15 peptide complex shows the <scene name='43/439961/Cv/4'>peptide passes through the PCNA ring</scene> and has <scene name='43/439961/Cv/3'>numerous interactions with it</scene><ref>PMID:25762514</ref>.
+p15 regulates DNA replication and repair by binding to PCNA.  PCNA-p15 peptide complex shows the <scene name='43/439961/Cv/5'>peptide passes through the PCNA ring</scene> and has <scene name='43/439961/Cv/6'>numerous interactions with it</scene><ref>PMID:25762514</ref>.
 </StructureSection>