Isopenicillin N synthase: Difference between revisions

Revision as of 13:21, 17 November 2016

Function

Isopenicillin N synthase (IPNS) is an iron-dependent enzyme which catalyzes the formation of isopenicillin N (IPN) from the tripeptide aminoadipoyl-cysteine-valine (ACV). IPNS participates in the biosynthesis of penicillin and cephalosporin antibiotics. The active site of IPNS contains an Fe atom. The reaction involves the reduction of O2 molecule to H2O^[1].

Structural highlights

IPNS . The ^[2]. Water molecules shown as red spheres.

3D structures of isopenicillin N synthase3D structures of isopenicillin N synthase

Updated on 17-November-2016

ReferencesReferences

↑ Burzlaff NI, Rutledge PJ, Clifton IJ, Hensgens CM, Pickford M, Adlington RM, Roach PL, Baldwin JE. The reaction cycle of isopenicillin N synthase observed by X-ray diffraction. Nature. 1999 Oct 14;401(6754):721-4. PMID:10537113 doi:10.1038/44400
↑ Roach PL, Clifton IJ, Hensgens CM, Shibata N, Schofield CJ, Hajdu J, Baldwin JE. Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation. Nature. 1997 Jun 19;387(6635):827-30. PMID:9194566 doi:http://dx.doi.org/10.1038/42990

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

[1] Burzlaff NI, Rutledge PJ, Clifton IJ, Hensgens CM, Pickford M, Adlington RM, Roach PL, Baldwin JE. The reaction cycle of isopenicillin N synthase observed by X-ray diffraction. Nature. 1999 Oct 14;401(6754):721-4. PMID:10537113 doi:10.1038/44400

[2] Roach PL, Clifton IJ, Hensgens CM, Shibata N, Schofield CJ, Hajdu J, Baldwin JE. Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation. Nature. 1997 Jun 19;387(6635):827-30. PMID:9194566 doi:http://dx.doi.org/10.1038/42990

[1]

[2]

@@ Line 1: / Line 1: @@
-<StructureSection load='1blz' size='400' side='right' scene='52/525170/Cv/1' caption='Isopenicilln N synthase complex with ACV and NO, [[1blz]]'>
+<StructureSection load='1blz' size='350' side='right' scene='52/525170/Cv/1' caption='Isopenicilln N synthase complex with ACV and NO, [[1blz]]'>
 == Function ==
@@ Line 15: / Line 15: @@
 **[[1bk0]] - AnIPNS + ACV – ''Aspergillus nidulans''<br />
 **[[2bjs]] - AnIPNS (mutant) + ACV<br />
-**[[1qiq]], [[1qjf]], [[1hb1]], [[1hb2]], [[1hb3]], [[1hb4]], [[1odm]], [[1odn]], [[1oc1]], [[1uzw]], [[1w03]], [[1w05]], [[2bu9]], [[1w3v]], [[1w3x]], [[2ivi]], [[2ivj]], [[2vau]], [[2vbb]], [[2vbd]], [[2vbp]], [[2vcm]], [[2ve1]], [[2wo7]], [[2y6f]], [[2y60]], [[2y86]], [[4bb3]] - AnIPNS + ACV derivative<br />
+**[[1qiq]], [[1qjf]], [[1hb1]], [[1hb2]], [[1hb3]], [[1hb4]], [[1odm]], [[1odn]], [[1oc1]], [[1uzw]], [[1w03]], [[1w05]], [[2bu9]], [[1w3v]], [[1w3x]], [[2ivi]], [[2ivj]], [[2vau]], [[2vbb]], [[2vbd]], [[2vbp]], [[2vcm]], [[2ve1]], [[2wo7]], [[2y6f]], [[2y60]], [[4bb3]] - AnIPNS + ACV derivative<br />
 **[[2jb4]] - AnIPNS + cyclobutanone analog<br />
 **[[1qje]] - AnIPNS + IPN + ACV<br />