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==NITRATE X-RAY INDUCED REDUCTION ON HEWL CRYSTALS (17.9 MGY)== | ==NITRATE X-RAY INDUCED REDUCTION ON HEWL CRYSTALS (17.9 MGY)== | ||
<StructureSection load='2ybl' size='340' side='right' caption='[[2ybl]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='2ybl' size='340' side='right' caption='[[2ybl]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2ybl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2ybl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YBL FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO2:NITRITE+ION'>NO2</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO2:NITRITE+ION'>NO2</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1w6z|1w6z]], [[1kxx|1kxx]], [[4lyo|4lyo]], [[3lyo|3lyo]], [[1kip|1kip]], [[1t6v|1t6v]], [[1ic7|1ic7]], [[1vds|1vds]], [[1lzt|1lzt]], [[1kir|1kir]], [[2xbr|2xbr]], [[1lys|1lys]], [[1bwj|1bwj]], [[132l|132l]], [[1e8l|1e8l]], [[1yil|1yil]], [[1heo|1heo]], [[1sfg|1sfg]], [[1kxw|1kxw]], [[2x0a|2x0a]], [[2c8o|2c8o]], [[1yl1|1yl1]], [[1sf4|1sf4]], [[1g7l|1g7l]], [[1ior|1ior]], [[1h87|1h87]], [[3lyt|3lyt]], [[1ljg|1ljg]], [[1dpx|1dpx]], [[1iot|1iot]], [[1v7s|1v7s]], [[1ja6|1ja6]], [[1jis|1jis]], [[1ir8|1ir8]], [[2w1m|2w1m]], [[1uic|1uic]], [[1xgq|1xgq]], [[1ykz|1ykz]], [[1uie|1uie]], [[2war|2war]], [[1lji|1lji]], [[8lyz|8lyz]], [[1lj3|1lj3]], [[1dpw|1dpw]], [[1bwi|1bwi]], [[2iff|2iff]], [[2lyo|2lyo]], [[1g7h|1g7h]], [[1lks|1lks]], [[1jj0|1jj0]], [[1rfp|1rfp]], [[5lyt|5lyt]], [[1sfb|1sfb]], [[1jiy|1jiy]], [[1iee|1iee]], [[1xei|1xei]], [[1ir7|1ir7]], [[1xek|1xek]], [[1hel|1hel]], [[1at6|1at6]], [[1ljf|1ljf]], [[1mlc|1mlc]], [[1f10|1f10]], [[2b5z|2b5z]], [[193l|193l]], [[1lsz|1lsz]], [[1ljk|1ljk]], [[6lyt|6lyt]], [[1sq2|1sq2]], [[1vdq|1vdq]], [[1zmy|1zmy]], [[2d91|2d91]], [[1lje|1lje]], [[2xth|2xth]], [[1b2k|1b2k]], [[1lze|1lze]], [[1aki|1aki]], [[1hen|1hen]], [[1uia|1uia]], [[1yik|1yik]], [[1xfp|1xfp]], [[2d6b|2d6b]], [[1lpi|1lpi]], [[1ndg|1ndg]], [[1flw|1flw]], [[1lsd|1lsd]], [[2blx|2blx]], [[6lyz|6lyz]], [[1nbz|1nbz]], [[1lsg|1lsg]], [[4lyt|4lyt]], [[1ved|1ved]], [[3hfm|3hfm]], [[1jit|1jit]], [[1lzn|1lzn]], [[1lyz|1lyz]], [[1wtn|1wtn]], [[1ja2|1ja2]], [[1uuz|1uuz]], [[2xbs|2xbs]], [[2d4i|2d4i]], [[2fbb|2fbb]], [[2lym|2lym]], [[1fdl|1fdl]], [[1gxx|1gxx]], [[1lz9|1lz9]], [[1lse|1lse]], [[1lzh|1lzh]], [[1lsm|1lsm]], [[3lym|3lym]], [[7lyz|7lyz]], [[1jj3|1jj3]], [[1yky|1yky]], [[1t3p|1t3p]], [[1heq|1heq]], [[1kiq|1kiq]], [[2lzh|2lzh]], [[1uih|1uih]], [[1kxy|1kxy]], [[2w1l|2w1l]], [[2bly|2bly]], [[1b0d|1b0d]], [[1g7j|1g7j]], [[1bhz|1bhz]], [[1her|1her]], [[1wtm|1wtm]], [[1hep|1hep]], [[1ioq|1ioq]], [[1nby|1nby]], [[1jtt|1jtt]], [[1qio|1qio]], [[1lza|1lza]], [[1xgp|1xgp]], [[1ps5|1ps5]], [[1gwd|1gwd]], [[1v7t|1v7t]], [[1jpo|1jpo]], [[1h6m|1h6m]], [[2a7d|2a7d]], [[1j1p|1j1p]], [[1dqj|1dqj]], [[2c8p|2c8p]], [[1z55|1z55]], [[1ljj|1ljj]], [[1lsb|1lsb]], [[1f0w|1f0w]], [[2w1x|2w1x]], [[1lzg|1lzg]], [[1flq|1flq]], [[1lzc|1lzc]], [[1jj1|1jj1]], [[1rcm|1rcm]], [[1uid|1uid]], [[1yqv|1yqv]], [[1hsx|1hsx]], [[1bgi|1bgi]], [[1lcn|1lcn]], [[1lzd|1lzd]], [[1hew|1hew]], [[2vb1|2vb1]], [[2cds|2cds]], [[2aub|2aub]], [[1hf4|1hf4]], [[1uib|1uib]], [[1ir9|1ir9]], [[2cgi|2cgi]], [[1rjc|1rjc]], [[1ios|1ios]], [[1j1x|1j1x]], [[1uc0|1uc0]], [[1azf|1azf]], [[1ljh|1ljh]], [[1ic4|1ic4]], [[4lyz|4lyz]], [[1gpq|1gpq]], [[2a6u|2a6u]], [[2d4k|2d4k]], [[1xej|1xej]], [[1ja7|1ja7]], [[1mel|1mel]], [[1ri8|1ri8]], [[1bvx|1bvx]], [[1uig|1uig]], [[1c10|1c10]], [[1qtk|1qtk]], [[1lkr|1lkr]], [[1lyo|1lyo]], [[2xjw|2xjw]], [[1hsw|1hsw]], [[1n4f|1n4f]], [[1jto|1jto]], [[1g7m|1g7m]], [[2w1y|2w1y]], [[1sf7|1sf7]], [[1lsf|1lsf]], [[1fn5|1fn5]], [[5lyz|5lyz]], [[2d4j|2d4j]], [[1c08|1c08]], [[3lzt|3lzt]], [[1ndm|1ndm]], [[1sf6|1sf6]], [[3lyz|3lyz]], [[1bvk|1bvk]], [[1uif|1uif]], [[2lyz|2lyz]], [[1vau|1vau]], [[1fly|1fly]], [[1lma|1lma]], [[1yl0|1yl0]], [[1j1o|1j1o]], [[1hc0|1hc0]], [[2lzt|2lzt]], [[4lzt|4lzt]], [[1a2y|1a2y]], [[1uco|1uco]], [[1lsy|1lsy]], [[5lym|5lym]], [[1lsa|1lsa]], [[1ic5|1ic5]], [[1p2c|1p2c]], [[1gxv|1gxv]], [[1ua6|1ua6]], [[1at5|1at5]], [[1hem|1hem]], [[1f3j|1f3j]], [[1vat|1vat]], [[1lj4|1lj4]], [[1vfb|1vfb]], [[1ja4|1ja4]], [[4lym|4lym]], [[2a7f|2a7f]], [[1flu|1flu]], [[194l|194l]], [[1lz8|1lz8]], [[1ykx|1ykx]], [[1bwh|1bwh]], [[1vdt|1vdt]], [[2hfm|2hfm]], [[1io5|1io5]], [[1lsn|1lsn]], [[1g7i|1g7i]], [[1lzb|1lzb]], [[2bpu|2bpu]], [[1lsc|1lsc]], [[1vdp|1vdp]], [[2ybn|2ybn]], [[2ybi|2ybi]], [[2ybh|2ybh]], [[2ybk|2ybk]], [[2ybm|2ybm]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1w6z|1w6z]], [[1kxx|1kxx]], [[4lyo|4lyo]], [[3lyo|3lyo]], [[1kip|1kip]], [[1t6v|1t6v]], [[1ic7|1ic7]], [[1vds|1vds]], [[1lzt|1lzt]], [[1kir|1kir]], [[2xbr|2xbr]], [[1lys|1lys]], [[1bwj|1bwj]], [[132l|132l]], [[1e8l|1e8l]], [[1yil|1yil]], [[1heo|1heo]], [[1sfg|1sfg]], [[1kxw|1kxw]], [[2x0a|2x0a]], [[2c8o|2c8o]], [[1yl1|1yl1]], [[1sf4|1sf4]], [[1g7l|1g7l]], [[1ior|1ior]], [[1h87|1h87]], [[3lyt|3lyt]], [[1ljg|1ljg]], [[1dpx|1dpx]], [[1iot|1iot]], [[1v7s|1v7s]], [[1ja6|1ja6]], [[1jis|1jis]], [[1ir8|1ir8]], [[2w1m|2w1m]], [[1uic|1uic]], [[1xgq|1xgq]], [[1ykz|1ykz]], [[1uie|1uie]], [[2war|2war]], [[1lji|1lji]], [[8lyz|8lyz]], [[1lj3|1lj3]], [[1dpw|1dpw]], [[1bwi|1bwi]], [[2iff|2iff]], [[2lyo|2lyo]], [[1g7h|1g7h]], [[1lks|1lks]], [[1jj0|1jj0]], [[1rfp|1rfp]], [[5lyt|5lyt]], [[1sfb|1sfb]], [[1jiy|1jiy]], [[1iee|1iee]], [[1xei|1xei]], [[1ir7|1ir7]], [[1xek|1xek]], [[1hel|1hel]], [[1at6|1at6]], [[1ljf|1ljf]], [[1mlc|1mlc]], [[1f10|1f10]], [[2b5z|2b5z]], [[193l|193l]], [[1lsz|1lsz]], [[1ljk|1ljk]], [[6lyt|6lyt]], [[1sq2|1sq2]], [[1vdq|1vdq]], [[1zmy|1zmy]], [[2d91|2d91]], [[1lje|1lje]], [[2xth|2xth]], [[1b2k|1b2k]], [[1lze|1lze]], [[1aki|1aki]], [[1hen|1hen]], [[1uia|1uia]], [[1yik|1yik]], [[1xfp|1xfp]], [[2d6b|2d6b]], [[1lpi|1lpi]], [[1ndg|1ndg]], [[1flw|1flw]], [[1lsd|1lsd]], [[2blx|2blx]], [[6lyz|6lyz]], [[1nbz|1nbz]], [[1lsg|1lsg]], [[4lyt|4lyt]], [[1ved|1ved]], [[3hfm|3hfm]], [[1jit|1jit]], [[1lzn|1lzn]], [[1lyz|1lyz]], [[1wtn|1wtn]], [[1ja2|1ja2]], [[1uuz|1uuz]], [[2xbs|2xbs]], [[2d4i|2d4i]], [[2fbb|2fbb]], [[2lym|2lym]], [[1fdl|1fdl]], [[1gxx|1gxx]], [[1lz9|1lz9]], [[1lse|1lse]], [[1lzh|1lzh]], [[1lsm|1lsm]], [[3lym|3lym]], [[7lyz|7lyz]], [[1jj3|1jj3]], [[1yky|1yky]], [[1t3p|1t3p]], [[1heq|1heq]], [[1kiq|1kiq]], [[2lzh|2lzh]], [[1uih|1uih]], [[1kxy|1kxy]], [[2w1l|2w1l]], [[2bly|2bly]], [[1b0d|1b0d]], [[1g7j|1g7j]], [[1bhz|1bhz]], [[1her|1her]], [[1wtm|1wtm]], [[1hep|1hep]], [[1ioq|1ioq]], [[1nby|1nby]], [[1jtt|1jtt]], [[1qio|1qio]], [[1lza|1lza]], [[1xgp|1xgp]], [[1ps5|1ps5]], [[1gwd|1gwd]], [[1v7t|1v7t]], [[1jpo|1jpo]], [[1h6m|1h6m]], [[2a7d|2a7d]], [[1j1p|1j1p]], [[1dqj|1dqj]], [[2c8p|2c8p]], [[1z55|1z55]], [[1ljj|1ljj]], [[1lsb|1lsb]], [[1f0w|1f0w]], [[2w1x|2w1x]], [[1lzg|1lzg]], [[1flq|1flq]], [[1lzc|1lzc]], [[1jj1|1jj1]], [[1rcm|1rcm]], [[1uid|1uid]], [[1yqv|1yqv]], [[1hsx|1hsx]], [[1bgi|1bgi]], [[1lcn|1lcn]], [[1lzd|1lzd]], [[1hew|1hew]], [[2vb1|2vb1]], [[2cds|2cds]], [[2aub|2aub]], [[1hf4|1hf4]], [[1uib|1uib]], [[1ir9|1ir9]], [[2cgi|2cgi]], [[1rjc|1rjc]], [[1ios|1ios]], [[1j1x|1j1x]], [[1uc0|1uc0]], [[1azf|1azf]], [[1ljh|1ljh]], [[1ic4|1ic4]], [[4lyz|4lyz]], [[1gpq|1gpq]], [[2a6u|2a6u]], [[2d4k|2d4k]], [[1xej|1xej]], [[1ja7|1ja7]], [[1mel|1mel]], [[1ri8|1ri8]], [[1bvx|1bvx]], [[1uig|1uig]], [[1c10|1c10]], [[1qtk|1qtk]], [[1lkr|1lkr]], [[1lyo|1lyo]], [[2xjw|2xjw]], [[1hsw|1hsw]], [[1n4f|1n4f]], [[1jto|1jto]], [[1g7m|1g7m]], [[2w1y|2w1y]], [[1sf7|1sf7]], [[1lsf|1lsf]], [[1fn5|1fn5]], [[5lyz|5lyz]], [[2d4j|2d4j]], [[1c08|1c08]], [[3lzt|3lzt]], [[1ndm|1ndm]], [[1sf6|1sf6]], [[3lyz|3lyz]], [[1bvk|1bvk]], [[1uif|1uif]], [[2lyz|2lyz]], [[1vau|1vau]], [[1fly|1fly]], [[1lma|1lma]], [[1yl0|1yl0]], [[1j1o|1j1o]], [[1hc0|1hc0]], [[2lzt|2lzt]], [[4lzt|4lzt]], [[1a2y|1a2y]], [[1uco|1uco]], [[1lsy|1lsy]], [[5lym|5lym]], [[1lsa|1lsa]], [[1ic5|1ic5]], [[1p2c|1p2c]], [[1gxv|1gxv]], [[1ua6|1ua6]], [[1at5|1at5]], [[1hem|1hem]], [[1f3j|1f3j]], [[1vat|1vat]], [[1lj4|1lj4]], [[1vfb|1vfb]], [[1ja4|1ja4]], [[4lym|4lym]], [[2a7f|2a7f]], [[1flu|1flu]], [[194l|194l]], [[1lz8|1lz8]], [[1ykx|1ykx]], [[1bwh|1bwh]], [[1vdt|1vdt]], [[2hfm|2hfm]], [[1io5|1io5]], [[1lsn|1lsn]], [[1g7i|1g7i]], [[1lzb|1lzb]], [[2bpu|2bpu]], [[1lsc|1lsc]], [[1vdp|1vdp]], [[2ybn|2ybn]], [[2ybi|2ybi]], [[2ybh|2ybh]], [[2ybk|2ybk]], [[2ybm|2ybm]]</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ybl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ybl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ybl RCSB], [http://www.ebi.ac.uk/pdbsum/2ybl PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ybl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ybl OCA], [http://pdbe.org/2ybl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ybl RCSB], [http://www.ebi.ac.uk/pdbsum/2ybl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ybl ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
Line 18: | Line 19: | ||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 2ybl" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
Line 25: | Line 27: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Chick]] | ||
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
[[Category: Carmichaael, I]] | [[Category: Carmichaael, I]] |
Revision as of 14:50, 5 August 2016
NITRATE X-RAY INDUCED REDUCTION ON HEWL CRYSTALS (17.9 MGY)NITRATE X-RAY INDUCED REDUCTION ON HEWL CRYSTALS (17.9 MGY)
Structural highlightsFunction[LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1] Publication Abstract from PubMedThe rate of radiation damage to macromolecular crystals at both room temperature and 100 K has previously been shown to be reduced by the use of certain radical scavengers. Here the effects of sodium nitrate, an electron scavenger, are investigated at 100 K. For sodium nitrate at a concentration of 0.5 M in chicken egg-white lysozyme crystals, the dose tolerance is increased by a factor of two as judged from the global damage parameters, and no specific structural damage to the disulfide bonds is seen until the dose is greatly in excess (more than a factor of five) of the value at which damage appears in electron density maps derived from a scavenger-free crystal. In the electron density maps, ordered nitrate ions adjacent to the disulfide bonds are seen to lose an O atom, and appear to protect the disulfide bonds. In addition, results reinforcing previous reports on the effectiveness of ascorbate are presented. The mechanisms of action of both scavengers in the crystalline environment are elucidated. Effective scavenging at cryotemperatures: further increasing the dose tolerance of protein crystals.,De la Mora E, Carmichael I, Garman EF J Synchrotron Radiat. 2011 May;18(Pt 3):346-57. Epub 2011 Apr 1. PMID:21525642[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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