Proteasome

File:3gpt.jpg

Crystal Structure of Proteasome, 3gpt

Proteasome (PRTS) are large protein complexes which degrade unneeded proteins into small polypeptides. The 26S PRTS is composed of a central 20S core particle which contains 4 stacked rings each with several members and two 19S caps. The core particle two outer rings contain 7 alpha subunits which form the PRTS gate. The two inner rings contain 6 beta subunits with protease activity. The 19S cap is composed of a base with 10 proteins six of which are ATPases and a lid which contains 9 proteins which binds polyubiquitin. The images at the left and at the right correspond to one representative Proteasome, i.e. the crystal structure of yeast Proteasome (3gpt).

3D Structures of Proteasome3D Structures of Proteasome

Update June 2011

20S particle20S particle

3mfe, 3mi0, 2fhg – Mt20S core – Mycobacterium tuberculosis
3gpt, 3gpw, 1g0u – y20S core - yeast
1ryp - y20S core (mutant)
2zcy – y20S+syringolin
3mka, 3hfa – Mt20S core (mutant)
3c91, 3c92, 1pma – Ta20S core - Themoplasma acidophilum
3h4p – 20S core – Methanocaldococcus janaschii
2h6j, 1q5r – Re20S core (mutant) – Rhodococcus erythropolis
1q5q - Re20S core
1j2q – Af20S core - Archaeoglobus fulgidus
1iru – 20S core - bovine

20S+activator protein20S+activator protein

1vsy, 1l5q – y20S core+ Blm10
1z7q, 1fnt – y20S core+PA26
3ipm, 3jrm, 3jse, 3jtl, 1ya7, 1yar, 1yau - Taa-PRTS chains A-G+ Tab-PRTS chains H-N+PA26 chains O-U

20S+inhibitor20S+inhibitor

3hye, 3gpj, 3dy3, 3dy4, 3e47, 3d29, 3bdm, 2zcy, 2gpl, 2fak, 2f16, 1jd2, 1g65, 3mg0, 3mg4, 3mg6, 3mg7, 3mg8 – y20S core+inhibitor
3h6f, 3h6i, 3hf9, 2fhh, 3krd – Mt20S core+inhibitor
3nzj, 3nzw, 3nzx - y20S + TMC-95A mimic ligand

Partial 20SPartial 20S

2ku1, 2ku2 – Taa-PRTS chains A-G
3fp9 – MtATPase chains A-L
1j2p – Af α subunit
2jay – Mt β subunit
2kqz, 2kr0 – hPRTS ubiquitin receptor domain

Partial 26SPartial 26S

1p9c – hPRTS subunit 4 C-terminal – human
1p9d - hPRTS subunit 4 C-terminal + HHR23A ubiquitin-like domain
1uel - hPRTS subunit 4 C-terminal + HHR23A ubiquitin-like domain - NMR
1yx4 - hPRTS subunit 4 – NMR
1yx5, 1yx6, 2kde, 2kdf - hPRTS subunit 4+ubiquitin – NMR
2o95, 2o96 - hPRTS subunit 7
3kw6 - hPRTS subunit 8
2krk - hPRTS subunit 8 – NMR
1qym, 1uoh - hPRTS subunit 10
1tr4 - hPRTS subunit 10 – NMR
2dvw, 2dwz - mPRTS subunit 10 + hPRTS subunit 6B – mouse
3aji - mPRTS subunit 10 + subunit 4
2x5n - PRTS subunit RPN10 VWA domain – fission yeast
2z4d - yPRTS subunit RPN13
2z59 - mPRTS subunit RPN13+ubiquitin