Proteasome

File:3gpt.jpg

Crystal Structure of Proteasome, 3gpt

Proteasome (PRTS) are large protein complexes which degrade unneeded proteins into small polypeptides. The 26S PRTS is composed of a central 20S core particle which contains 4 stacked rings each with several members and two 19S caps. The core particle two outer rings contain 7 alpha subunits which form the PRTS gate. The two inner rings contain 6 beta subunits with protease activity. The 19S cap is composed of a base with 10 proteins six of which are ATPases and a lid which contains 9 proteins which binds polyubiquitin. The images at the left and at the right correspond to one representative Proteasome, i.e. the crystal structure of yeast Proteasome (3gpt).

3D Structures of Proteasome3D Structures of Proteasome

20S particle20S particle

3mfe, 3mi0, 2fhg – Mt20S core – Mycobacterium tuberculosis
3gpt, 3gpw, 1g0u – y20S core - yeast
1ryp - y20S core (mutant)
3mka, 3hfa – Mt20S core (mutant)
3c91, 3c92, 1pma – Ta20S core - Themoplasma acidophilum
3h4p – 20S core – Methanocaldococcus janaschii
2h6j, 1q5r – Re20S core (mutant) – Rhodococcus erythropolis
1q5q - Re20S core
1j2q – Af20S core - Archaeoglobus fulgidus
1iru – 20S core - bovine

20S+activator protein20S+activator protein

1vsy, 1l5q – y20S core+ Blm10
1z7q, 1fnt – y20S core+PA26
3ipm, 3jrm, 3jse, 3jtl, 1ya7, 1yar, 1yau - Taa-PRTS chains A-G+ Tab-PRTS chains H-N+PA26 chains O-U

20S+inhibitor20S+inhibitor

3hye, 3gpj, 3dy3, 3dy4, 3e47, 3d29, 3bdm, 2gpl, 2fak, 2f16, 1jd2, 1g65 – y20S core+inhibitor
3h6f, 3h6i, 3hf9, 2fhh – Mt20S core+inhibitor

Partial 20SPartial 20S

2ku1, 2ku2 – Taa-PRTS chains A-G
3fp9 – MtATPase chains A-L
1j2p – Af α subunit