Invertase: Difference between revisions
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== Function == | == Function == | ||
'''Invertase''' (INV) catalyzes the hydrolysis of sucrose into glucose and fructose<ref>PMID:4963242</ref>. INV is produced by bees which use it to make honey from nectar. | '''Invertase''' (INV) catalyzes the hydrolysis of sucrose into glucose and fructose<ref>PMID:4963242</ref>. INV is produced by bees which use it to make honey from nectar. | ||
*'''Cell-wall INV''' is crucial for metabolism, growth and differentiation in plants<ref>PMID:24646208</ref>. | |||
*'''Extracellular INV''' catalyzes the cleavage of the transport sugar sucrose released into the app-last<ref>PMID:12508062</ref> | |||
*'''Alkaline INV''' is a tetramer which catalyzes the cleavage of sucrose<ref>PMID:8972597</ref> | |||
*'''DNA INV Gin''' is found in bacteriophage Mu and catalyses the recombination between repeat sequences in vivo and in vitro<ref>PMID:3042382</ref> | |||
For details in Hebrew see [[Invertase (Hebrew)]]. | |||
== Relevance == | == Relevance == | ||
INV is used in the food industry to produce fructose which is sweeter and does not crystallize easily. | INV is used in the food industry to produce fructose which is sweeter and does not crystallize easily. | ||
== Structural highlights == | == Structural highlights == | ||
The <scene name='67/676994/Cv/ | The <scene name='67/676994/Cv/7'>active site pocket</scene> of INV contains the catalytic residues Asp and Glu<ref>PMID:16411890</ref>. | ||
</StructureSection> | </StructureSection> | ||
| Line 18: | Line 24: | ||
*Invertase | *Invertase | ||
**[[4eqv]] – INV 2 – yeast<br /> | |||
**[[1uyp]] – TmINV – ''Thermotoga maritima''<br /> | **[[1uyp]] – TmINV – ''Thermotoga maritima''<br /> | ||
**[[ | **[[1w2t]] – TmINV (mutant) + trisaccharide<br /> | ||
**[[3kf5]] – SoINV – ''Schwanniomyces occidentalis''<br /> | **[[3kf5]] – SoINV – ''Schwanniomyces occidentalis''<br /> | ||
**[[3kf3]] – SoINV + fructose <br /> | **[[3kf3]] – SoINV + fructose <br /> | ||
| Line 29: | Line 35: | ||
**[[2oxb]], [[2qqu]], [[2qqv]], [[2qqw]] – AtINV (mutant) + sucrose<br /> | **[[2oxb]], [[2qqu]], [[2qqv]], [[2qqw]] – AtINV (mutant) + sucrose<br /> | ||
**[[2xqr]] – AtINV + INV inhibitor <br /> | **[[2xqr]] – AtINV + INV inhibitor <br /> | ||
**[[6ttj]] – AtINV 2<br /> | |||
*Alkaline invertase | *Alkaline invertase | ||
**[[5gor]] – AnINV – ''Anabaena''<br /> | **[[5gor]], [[5z73]] – AnINV – ''Anabaena''<br /> | ||
**[[5goo]], [[5gop]], [[5goq]] – AnINV + sugar <br /> | **[[5goo]], [[5gop]], [[5goq]] – AnINV + sugar <br /> | ||
Latest revision as of 15:02, 7 July 2024
FunctionInvertase (INV) catalyzes the hydrolysis of sucrose into glucose and fructose[1]. INV is produced by bees which use it to make honey from nectar.
For details in Hebrew see Invertase (Hebrew). RelevanceINV is used in the food industry to produce fructose which is sweeter and does not crystallize easily. Structural highlightsThe of INV contains the catalytic residues Asp and Glu[6]. |
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3D Structures of invertase3D Structures of invertase
Updated on 07-July-2024
ReferencesReferences
- ↑ Neumann NP, Lampen JO. Purification and properties of yeast invertase. Biochemistry. 1967 Feb;6(2):468-75. PMID:4963242
- ↑ Proels RK, Huckelhoven R. Cell-wall invertases, key enzymes in the modulation of plant metabolism during defence responses. Mol Plant Pathol. 2014 Oct;15(8):858-64. doi: 10.1111/mpp.12139. Epub 2014 May, 13. PMID:24646208 doi:http://dx.doi.org/10.1111/mpp.12139
- ↑ Roitsch T, Balibrea ME, Hofmann M, Proels R, Sinha AK. Extracellular invertase: key metabolic enzyme and PR protein. J Exp Bot. 2003 Jan;54(382):513-24. PMID:12508062
- ↑ Lee HS, Sturm A. Purification and characterization of neutral and alkaline invertase from carrot. Plant Physiol. 1996 Dec;112(4):1513-22. PMID:8972597 doi:10.1104/pp.112.4.1513
- ↑ Klippel A, Mertens G, Patschinsky T, Kahmann R. The DNA invertase Gin of phage Mu: formation of a covalent complex with DNA via a phosphoserine at amino acid position 9. EMBO J. 1988 Apr;7(4):1229-37. PMID:3042382 doi:10.1002/j.1460-2075.1988.tb02935.x
- ↑ Alberto F, Jordi E, Henrissat B, Czjzek M. Crystal structure of inactivated Thermotoga maritima invertase in complex with the trisaccharide substrate raffinose. Biochem J. 2006 May 1;395(3):457-62. PMID:16411890 doi:http://dx.doi.org/BJ20051936