5z73

Crystal structure of alkaline/neutral invertase InvB from Anabaena sp. PCC 7120Crystal structure of alkaline/neutral invertase InvB from Anabaena sp. PCC 7120

Structural highlights 5z73 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NonStd Res: Activity: Beta-fructofuranosidase, with EC number 3.2.1.26 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Anabaena sp. PCC 7120 encodes two alkaline/neutral invertases, namely InvA and InvB. Following our recently reported InvA structure, here we report the crystal structure of the heterocyst-specific InvB. Despite sharing an overall structure similar to InvA, InvB possesses a much higher catalytic activity. Structural comparisons of the catalytic pockets reveal that Arg430 of InvB adopts a different conformation, which may facilitate the deprotonation of the catalytic residue Glu415. We propose that the higher activity may be responsible for the vital role of InvB in heterocyst development and nitrogen fixation. Furthermore, phylogenetic analysis combined with activity assays also suggests the role of this highly conserved arginine in plants and cyanobacteria, as well as some proteobacteria living in highly extreme environments. Structural and enzymatic analyses of Anabaena heterocyst-specific alkaline invertase InvB.,Xie J, Hu HX, Cai K, Xia LY, Yang F, Jiang YL, Chen Y, Zhou CZ FEBS Lett. 2018 May;592(9):1589-1601. doi: 10.1002/1873-3468.13041. Epub 2018 Apr, 10. PMID:29578606 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 5z73, resolution 1.93Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image