Transcriptional activator: Difference between revisions

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<StructureSection load='1h89' size='350' side='right' caption='Structure of transcriptional activator C-Myb DNA-binding domain (pink) complex with DNA, CAAT/enhancer binding protein β (grey and green) and K+ ion (PDB entry [[1h89]])' scene=''>
<StructureSection load='1h89' size='350' side='right' caption='Structure of transcriptional activator C-Myb DNA-binding domain (deeppink) complex with DNA, CAAT/enhancer binding protein β (cyan and green) and K+ ion (purple) (PDB entry [[1h89]])' scene='55/553105/Cv/1'>
 
__TOC__
'''Transcriptional activators''' (TA) are proteins which bind to DNA and stimulate nearby gene transcription.  TA transcription stimulation is achieved by enhancing RNA polymerase binding or by enhancing the formation of open complex which is required for initiation of transcription.  TA bind to a specific DNA sequence in or near an operon via their DNA-binding domain (DBD).
== Function == 
'''Transcriptional activators''' or '''Transcriptional regulators''' (TA) are proteins which bind to DNA and stimulate nearby gene transcription.  TA transcription stimulation is achieved by enhancing RNA polymerase binding or by enhancing the formation of open complex which is required for initiation of transcription.  TA bind to a specific DNA sequence in or near an operon via their DNA-binding domain (DBD)<ref>PMID:16464826</ref>. '''PrfA''' is an activator of virulence genes in ''Listeria monocytogenes''<ref>PMID:10564496</ref>. See also [[Listeriolysin positive regulatory factor A]].  


== Structural highlights ==
TA contains 3 domains: DBD, trans-activating domain (TAD) which binds coregulator proteins and an optional ligand-binding domain (LBD) which senses external signals.
*<scene name='55/553105/Cv/5'>Transcriptional activator C-Myb DNA-binding domain complex with DNA, CAAT/enhancer binding protein β and K+ ion </scene> (PDB entry [[1h89]]).
*<scene name='55/553105/Cv/6'>1st K soordination site</scene>.
*<scene name='55/553105/Cv/7'>2nd K soordination site</scene>. Water molecules are shown as red spheres.
== 3D Structures of transcriptional activator==
== 3D Structures of transcriptional activator==
[[Transcriptional activator 3D structures]]


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
</StructureSection>


'''Gcn4 TA'''
== References ==
 
<references/>
See [[Gcn4]]
 
'''C-Myb  TA'''
 
[[1mse]], [[1msf]] – TA DBD + DNA - NMR<br />
[[1mbe]], [[1mbf]] – mTA DBD repeat 1 + DNA – mouse - NMR<br />
[[1mbg]], [[1mbh]] – mTA DBD repeat 2 + DNA - NMR<br />
[[1mbj]], [[1mbk]] – mTA DBD repeat 3 + DNA - NMR<br />
[[1h88]] – mTA DBD + DNA + CCAAT/enhancer binding protein β<br />
[[1h89]] – mTA DBD + DNA + CAAT/enhancer binding protein β<br />
[[1guu]] – mTA DBD repeat 1  <br />
[[1gv2]] – mTA DBD repeat 2,3 <br />
[[1gv5]] – mTA DBD repeat 2  <br />
[[1gvd]] – mTA DBD repeat 2 (mutant) <br />
[[1sb0]], [[2agh]] – mTA transcription activation domain + CREB-binding protein - NMR<br />
 
'''Psp operon  TA'''
 
[[2bjw]] – EcTA AAA domain – ''Escherichia coli''<br />
[[2bjv]], [[2c9c]] – EcTA AAA domain (mutant)<br />
[[2c96]] – EcTA AAA domain + ATP<br />
[[2c98]] – EcTA AAA domain + ADP<br />
[[2c99]] – EcTA AAA domain + AMPPNP<br />
[[2vii]] – EcTA AAA domain + AMP + Mg<br />
 
'''TenA  TA'''
 
[[1tyh]], [[1yaf]] – BsTA – ''Bacillus subtilis''<br />
[[1yak]] – BsTA + pyrimidine derivative<br />
[[1qcx]] – BsTA (mutant) + pyrimidine derivative<br />
 
'''LasR  TA'''
 
[[2uv0]] – PaTA ligand-binding domain – ''Pseudomonas aeruginosa''<br />
[[3ix3]] – PaTA ligand-binding domain + oxo-dodecanoyl-homoserine lactone<br />
[[3ix3]], [[3ix8]], [[3jpu]] – PaTA ligand-binding domain + benzoate derivative<br />
 
'''CadC  TA'''
 
[[3ly7]], [[3lya]] – EcTA periplasmic domain <br />
[[3ly8]], [[3ly9]] – EcTA periplasmic domain (mutant)<br />
 
'''CynR  TA'''
 
[[2hxr]] – EcTA ligand-binding domain <br />
[[3hfu]] – EcTA ligand-binding domain + N3<br />
 
'''Mal-Rpel  TA'''
 
[[2v51]], [[2v52]] – mTA residues 16-41 + actin <br />
[[2yje]], [[2yjf]] – mTA Rpel domain + actin <br />
 
'''Brg1  TA'''
 
[[3uvd]] – TA bromodomain + pyrrolidone derivative - human<br />
 
'''VP30  TA'''
 
[[2i8b]] – TA C terminal – Zaire ebolavirus<br />
[[3v7o]] – TA C terminal – Reston ebolavirus<br />
[[Category:Topic Page]]
[[Category:Topic Page]]

Latest revision as of 09:48, 20 August 2024

Function

Transcriptional activators or Transcriptional regulators (TA) are proteins which bind to DNA and stimulate nearby gene transcription. TA transcription stimulation is achieved by enhancing RNA polymerase binding or by enhancing the formation of open complex which is required for initiation of transcription. TA bind to a specific DNA sequence in or near an operon via their DNA-binding domain (DBD)[1]. PrfA is an activator of virulence genes in Listeria monocytogenes[2]. See also Listeriolysin positive regulatory factor A.

Structural highlights

TA contains 3 domains: DBD, trans-activating domain (TAD) which binds coregulator proteins and an optional ligand-binding domain (LBD) which senses external signals.

  • (PDB entry 1h89).
  • .
  • . Water molecules are shown as red spheres.

3D Structures of transcriptional activator

Transcriptional activator 3D structures


Structure of transcriptional activator C-Myb DNA-binding domain (deeppink) complex with DNA, CAAT/enhancer binding protein β (cyan and green) and K+ ion (purple) (PDB entry 1h89)

Drag the structure with the mouse to rotate

ReferencesReferences

  1. ↑ Titz B, Thomas S, Rajagopala SV, Chiba T, Ito T, Uetz P. Transcriptional activators in yeast. Nucleic Acids Res. 2006 Feb 7;34(3):955-67. Print 2006. PMID:16464826 doi:http://dx.doi.org/34/3/955
  2. ↑ Renzoni A, Cossart P, Dramsi S. PrfA, the transcriptional activator of virulence genes, is upregulated during interaction of Listeria monocytogenes with mammalian cells and in eukaryotic cell extracts. Mol Microbiol. 1999 Nov;34(3):552-61. doi: 10.1046/j.1365-2958.1999.01621.x. PMID:10564496 doi:http://dx.doi.org/10.1046/j.1365-2958.1999.01621.x

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Michal Harel, Alexander Berchansky
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