DNA adenine methylase

Function

DNA adenine methylase (DAM) adds a methyl group to adenines in the DNA sequence GATC. The daughter strand remains unmethylated for a short while during which mismatch repair of DNA can take place. DAM has a role in regulation of replication by delaying the availability of the origin of replication to activation during the cell cycle. The origin of replication in E. coli contains 11 GATC sequences. In E. coli, downstream GATC sequences are methylated to promote RNA transcription. ^[1]

Structural highlights

DAM uses S-adenosyl-L-homocysteine (AdoHcy, SAH) as methyl donor.

(PDB code 1q0t) ^[2]

Phage T4 DNA adenine metylase complex with SAH, DNA and I- ion (purple) (PDB code 1q0t)

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3D Structures of DNA adenine methylase3D Structures of DNA adenine methylase

Updated on 25-February-2019

1q0s – T4DAM + SAH – Enterobacter phage T4
1q0t, 1yf3, 1yfj – T4DAM + SAH + DNA
1yfl – T4DAM + sinefungin + DNA
2g1p, 4rtk, 4rtn, 4rtq – EcDAM + SAH + DNA – Escherichia coli
2ore, 4gbe – EcDAM + SAH
4gol, 4gom, 4gon, 4goo – EcDAM + Aza-SAM derivative
4rtj, 4rtl, 4rto – EcDAM + sinefungin + DNA
4rtm, 4rtp, 4rtr, 4rts – EcDAM + AdoMet + DNA

ReferencesReferences

↑ Lobner-Olesen A, Skovgaard O, Marinus MG. Dam methylation: coordinating cellular processes. Curr Opin Microbiol. 2005 Apr;8(2):154-60. PMID:15802246 doi:http://dx.doi.org/10.1016/j.mib.2005.02.009
↑ Yang Z, Horton JR, Zhou L, Zhang XJ, Dong A, Zhang X, Schlagman SL, Kossykh V, Hattman S, Cheng X. Structure of the bacteriophage T4 DNA adenine methyltransferase. Nat Struct Biol. 2003 Oct;10(10):849-55. Epub 2003 Aug 24. PMID:12937411 doi:http://dx.doi.org/10.1038/nsb973

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Alexander Berchansky, Michal Harel, Joel L. Sussman

[1] Lobner-Olesen A, Skovgaard O, Marinus MG. Dam methylation: coordinating cellular processes. Curr Opin Microbiol. 2005 Apr;8(2):154-60. PMID:15802246 doi:http://dx.doi.org/10.1016/j.mib.2005.02.009

[2] Yang Z, Horton JR, Zhou L, Zhang XJ, Dong A, Zhang X, Schlagman SL, Kossykh V, Hattman S, Cheng X. Structure of the bacteriophage T4 DNA adenine methyltransferase. Nat Struct Biol. 2003 Oct;10(10):849-55. Epub 2003 Aug 24. PMID:12937411 doi:http://dx.doi.org/10.1038/nsb973

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