Catabolite gene activator protein
Catabolite gene activator protein (CAP) is a DNA-binding protein involved in the transcription of several genes including those involved in the metabolism of sugar. CAP can activate or repress transcription initiation upon binding of cAMP. The cAMP-CAP complex binds to promoters on the DNA. The structure contains a ligand-binding domain (LBD) at the N-terminal and DNA-binding domain (DBD) at the C-terminal. . When the amount of glucose transported into the cell is low, cAMP level rises and the cAMP-CAP complex causes increase of transcription of glucose metabolism genes. [1] See also CAMP-dependent pathway and CAMP is second messenger.
in E. coli Catabolite gene activator protein (1o3r). [2] Water molecules are shown as red spheres. 3D Structures of catabolite gene activator proteinCatabolite gene activator protein 3D structures
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ReferencesReferences
- ↑ Busby S, Ebright RH. Transcription activation by catabolite activator protein (CAP). J Mol Biol. 1999 Oct 22;293(2):199-213. PMID:10550204 doi:http://dx.doi.org/10.1006/jmbi.1999.3161
- ↑ Chen S, Vojtechovsky J, Parkinson GN, Ebright RH, Berman HM. Indirect readout of DNA sequence at the primary-kink site in the CAP-DNA complex: DNA binding specificity based on energetics of DNA kinking. J Mol Biol. 2001 Nov 16;314(1):63-74. PMID:11724532 doi:10.1006/jmbi.2001.5089