7dfr
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CRYSTAL STRUCTURES OF ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE. THE NADP+ HOLOENZYME AND THE FOLATE(DOT)NADP+ TERNARY COMPLEX. SUBSTRATE BINDING AND A MODEL FOR THE TRANSITION STATE
OverviewOverview
The crystal structure of dihydrofolate reductase (EC 1.5.1.3) from, Escherichia coli has been solved as the binary complex with NADP+ (the, holoenzyme) and as the ternary complex with NADP+ and folate. The Bragg, law resolutions of the structures are 2.4 and 2.5 A, respectively. The new, crystal forms are nonisomorphous with each other and with the methotrexate, binary complex reported earlier [Bolin, J. T., Filman, D. J., Matthews, D., A., Hamlin, R. C., & Kraut, J. (1982) J. Biol. Chem. 257, 13650-13662]. In, general, NADP+ and folate binding conform to predictions, but the, nicotinamide moiety of NADP+ is disordered in the holoenzyme and ordered, in the ternary complex. A mobile loop (residues 16-20) involved in binding, the nicotinamide is also disordered in the holoenzyme. We report a, detailed analysis of the binding interactions for both ligands, paying, special attention to several apparently strained interactions that may, favor the transition state for hydride transfer. Hypothetical models are, presented for the binding of 7,8-dihydrofolate in the Michaelis complex, and for the transition-state complex.
About this StructureAbout this Structure
7DFR is a Single protein structure of sequence from Escherichia coli with FOL and NAP as ligands. The following page contains interesting information on the relation of 7DFR with [Dihydrofolate Reductase]. Active as Dihydrofolate reductase, with EC number 1.5.1.3 Full crystallographic information is available from OCA.
ReferenceReference
Crystal structures of Escherichia coli dihydrofolate reductase: the NADP+ holoenzyme and the folate.NADP+ ternary complex. Substrate binding and a model for the transition state., Bystroff C, Oatley SJ, Kraut J, Biochemistry. 1990 Apr 3;29(13):3263-77. PMID:2185835
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