Crystal structure of the ternary complex of yeast tyrosyl-tRNA synthetaseCrystal structure of the ternary complex of yeast tyrosyl-tRNA synthetase
Structural highlights
2dlc is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
SYYC_YEAST Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). The specificity determinants on tRNA(Tyr) are the base pair C1-G72, the discriminator residue A73, and the three anticodon bases G34, U35 and A36. Also involved in nuclear tRNA export.[1][2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
↑Sarkar S, Azad AK, Hopper AK. Nuclear tRNA aminoacylation and its role in nuclear export of endogenous tRNAs in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1999 Dec 7;96(25):14366-71. PMID:10588711
↑Fechter P, Rudinger-Thirion J, Theobald-Dietrich A, Giege R. Identity of tRNA for yeast tyrosyl-tRNA synthetase: tyrosylation is more sensitive to identity nucleotides than to structural features. Biochemistry. 2000 Feb 22;39(7):1725-33. PMID:10677221
