3lha
Crystal structure of mouse VPS26B(R240S/G241A/E242S) in spacegroup P41 21 2Crystal structure of mouse VPS26B(R240S/G241A/E242S) in spacegroup P41 21 2
Structural highlights
FunctionVP26B_MOUSE Probable component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedRetromer is a peripheral membrane protein complex that has pleiotropic roles in endosomal membrane trafficking. The core of retromer possesses three subunits VPS35, VPS29 and VPS26 that play different roles in binding to cargo, regulatory proteins and complex stabilisation. We have performed an investigation of the thermodynamics of core retromer assembly using isothermal titration calorimetry (ITC) demonstrating that VPS35 acts as the central subunit to which VPS29 and VPS26 bind independently. Furthermore we confirm that the conserved PRLYL motif of the large VPS35 subunit is critical for direct VPS26 interaction. Heat capacity measurements of VPS29 and VPS26 binding to VPS35 indicate extensive binding interfaces and suggest conformational alterations in VPS29 or VPS35 upon complex formation. Solution studies of the retromer core using small angle X-ray scattering, allow us to propose a model whereby VPS35 forms an extended platform with VPS29 and VPS26 bound at distal ends, with the potential for forming dimeric assemblies. Assembly and solution structure of the core retromer protein complex.,Norwood SJ, Shaw DJ, Cowieson NP, Owen DJ, Teasdale RD, Collins BM Traffic. 2010 Sep 28. doi: 10.1111/j.1600-0854.2010.01124.x. PMID:20875039[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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