Nitrate reductase

Function

Nitrate reductase (NR) catalyzes the reduction of NO₃ to NO₂ using NADPH^[1]. NR active site contains Mo atom. Four types of NR are known:

eukaryotic assimilatory NR
bacterial cytoplasmic assimilatory (NAC)
bacterial membrane-bound respiratory (NAR)
bacterial periplasmic dissimilatory (NAP).
The cofactors of NR are Molybdopterin (MPT) in eukaryotic NR and bis-Molybdopterin guanine dinucleotide (MGD) in bacterial NR.

Structural highlights

. NAP active site includes a . The ^[2].

Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo, NO3- and molybdepterin guanine nucleotide (PDB entry 2jiq)

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3D structures of nitrate reductase3D structures of nitrate reductase

Updated on 23-July-2019

ReferencesReferences

↑ NICHOLAS DJ, NASON A. Molybdenum and nitrate reductase. II. Molybdenum as a constituent of nitrate reductase. J Biol Chem. 1954 Mar;207(1):353-60. PMID:13152110
↑ Najmudin S, Gonzalez PJ, Trincao J, Coelho C, Mukhopadhyay A, Cerqueira NM, Romao CC, Moura I, Moura JJ, Brondino CD, Romao MJ. Periplasmic nitrate reductase revisited: a sulfur atom completes the sixth coordination of the catalytic molybdenum. J Biol Inorg Chem. 2008 Mar 8;. PMID:18327621 doi:10.1007/s00775-008-0359-6

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Michal Harel, Alexander Berchansky, Joel L. Sussman

[1] NICHOLAS DJ, NASON A. Molybdenum and nitrate reductase. II. Molybdenum as a constituent of nitrate reductase. J Biol Chem. 1954 Mar;207(1):353-60. PMID:13152110

[2] Najmudin S, Gonzalez PJ, Trincao J, Coelho C, Mukhopadhyay A, Cerqueira NM, Romao CC, Moura I, Moura JJ, Brondino CD, Romao MJ. Periplasmic nitrate reductase revisited: a sulfur atom completes the sixth coordination of the catalytic molybdenum. J Biol Inorg Chem. 2008 Mar 8;. PMID:18327621 doi:10.1007/s00775-008-0359-6

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