Bam complex

Function The Bam (β-Barrel Assembly Machinery) drives the assembly of β-barrel proteins into the outer membrane of gram-negative bacteria. Structural highlights The complex is composed of five subunits: BamA, BamB, BamC, BamD and BamE. Outer membrane β-barrel proteins assembly is dependent on Bam in various organisms. BamB,C,D,E bind to the N-terminal of BamA. BamA contains five structurally homologous POTRA (POlypeptide TRAnslocation associated) domains. The POTRA domain has a β-α-α-β-β conformation. BamA barrel and at least a subset of its POTRAs are essential for viability. BamA was found also in mitochondria and chloroplasts. BamD is composed of multiple tetratricopeptide (TPR) repeats packed into a superhelical structure. TPR is a motif containing 2 antiparallel α-helices. TPRs are found in scaffold multiprotein complexes and are involved in protein-protein interactions. 3D Structures of Bam complex Bam complex 3D structures

spinqualitylabelsall models E. coli BamA barrel domain (PDB code 4n75) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D Structures of Bam complex3D Structures of Bam complex

Updated on 01-April-2019

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• BamA (Omp85)
  • 4k3b – NgBamA – Neisseria gonorrhoeae
    
  • 4k3c – BamA – Haemophilus ducreyi
    
  • 5or1 – BamA – Salmonella typhimurium
    
  • 2qcz, 2qdf – EcBamA residues 21-351 – Escherichia coli
    
  • 3q6b – EcBamA POTRA1-2 domain residues 21-174
    
  • 3efc – EcBamA periplasmic domain residues 21-410
    
  • 4c4v – EcBamA residues 344-808
    
  • 4pk1 – EcBamA/BamB
    
  • 4n75, 6fsu – EcBamA barrel domain residues 427-810
    
  • 3og5, 3q6b – EcBamA POTRA45 domain residues 264-424
    
• BamB (Lipoprotein YFGL)
  • 3q7m, 3q7n, 3q7o, 3p1l, 2yms – EcBamB
    
  • 3q54, 3prw, 2yh3 – EcBamB residues 25-392
    
  • 4hdj – BamB residues 20-380 – Pseudomonas aeruginosa
    
  • 4imm – BamB – Moraxella catarrhalis
    
• BamC (Lipoprotein 34)
  • 2yh5, 3sns – EcBamC C terminal
    
  • 2lae – EcBamC C terminal - NMR
    
  • 2yh6 – EcBamC N terminal
    
  • 2laf – EcBamC N terminal - NMR
    
• BamD (Lipoprotein YFIO)
  • 2yhc, 3q5m – EcBamD residues 29-245
    
  • 3qky – RmBamD residues 24-280 – Rhodothermus marinus
    
  • 3tgo – EcBamD residues 21-245 + lipoprotein 34
    
  • 5waq – NgBamD
    
• BamE (Small protein A)
  • 2yh9 – EcBamE residues 34-113
    
  • 2kxx – EcBamE residues 21-114 - NMR
    
  • 2km7 – EcBamE residues 21-114 (mutant) - NMR
    
  • 5wam – NgBamE
    
• BamA+BamB
  • 4xga – EcBam
    
• BamA+BamD
  • 5efr – RmBam
    
• BamA+BamC+BamD+BamE
  • 5ekq, 5d0q – EcBam
    
• BamA+BamB+BamC+BamD+BamE
  • 5d0o, 5ayw – EcBam
    
  • 5ljo – EcBam – Cryo EM