FunctiontRNA pseudouridine synthase (PUS) catalyzes the conversion of tRNA uridine to tRNA pseudouridine[1]. PUS are active in the ribosomal large subunit Ribosomal large subunit pseudouridine synthase and in the ribosomal small subunit Ribosomal small subunit pseudouridine synthase. Ribosomal large subunit pseudouridine synthase RluD is responsible for synthesis of pseudouridine from uracil in positions 1911, 1915 and 1917 in 23S ribosomal RNA[2]. Probable tRNA pseudouridine synthase (PPUS) is a similar enzyme.
Structural highlightsThe conversion of uridine to pseudouridine causes conformational change of PUS[3]. (1ze2).
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3D Structures of tRNA pseudouridine synthase3D Structures of tRNA pseudouridine synthase
Updated on 12-November-2018
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- tRNA pseudouridine synthase
- 1dj0 – EcPUS I – Escherichia coli
- 4iqm, 4its, 4j37, 4nz6 - hPUS Pus1 catalytic domain - human
- 4nz7 - hPUS Pus1 catalytic domain (mutant)
- 1vs3 – PUS TruA – Thermus thermophilus
- 1sgv – PUS TruB – Mycobacterium tuberculosis
- 1si7, 1szw, 1sb7 - EcPUS TruD
- tRNA pseudouridine synthase complex with RNA
- 1k8w – EcTruB + stem-loop RNA
- 1zl3 - EcTruB (mutant) + stem-loop RNA
- 2nqp, 2nr0, 2nre – EcTruA + Leu-tRNA
- 1ze2 - TmPUS B + RNA
- 2ab4 - TmPUS B (mutant) + RNA
- 1r3e, 1r3f - EcTruB + RNA
- Probable tRNA pseudouridine synthase
- 3hjw, 3lwo, 3lwp, 3lwq, 3lwr, 3lwv, 3hax – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA – Pyrococcus furiosus
- 3hay – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA + small nuclear RNP protein
- 2hvy – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA + small nuclear RNP protein + small nuclear RNP Gar1-like protein
- 3mqk - PfPUS Cbf5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA
- 2rfk - PfPUS Cbf5 (mutant) + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA
- 2ey4 - PfPUS Cbf5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein
- 3hjy - PfPUS Cbf5 + ribosome biogenesis protein NOP10 + RNA
- 2apo - PfPUS Cbf5 + ribosome biogenesis protein NOP10 - Methanocaldococcus jannaschii
- Ribosomal large subunit pseudouridine synthase
- 2i82 - EcRluA catalytic domain + RNA
- 4lab - EcRluB catalytic domain
- 4lgt - EcRluB catalytic domain + RNA
- 1xpi - EcRluC catalytic domain
- 5vbb - hRluC-like
- 1prz, 1qyu, 1v9f, 1v9k, 2ist - EcRluD catalytic domain
- 2oml, 2olw - EcRluE catalytic domain
- 2gml - EcRluF catalytic domain
- 3dh3 - EcRluF catalytic domain + RNA
- Ribosomal small subunit pseudouridine synthase
- 1vio - RsuA - Haemophilus influenzae
- 1ksk - EcRsuA
- 1ksl - EcRsuA + uracil
- 1ksv - EcRsuA + UMP
ReferencesReferences
- ↑ Pan H, Agarwalla S, Moustakas DT, Finer-Moore J, Stroud RM. Structure of tRNA pseudouridine synthase TruB and its RNA complex: RNA recognition through a combination of rigid docking and induced fit. Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12648-53. Epub 2003 Oct 17. PMID:14566049 doi:10.1073/pnas.2135585100
- ↑ Gutgsell NS, Deutscher MP, Ofengand J. The pseudouridine synthase RluD is required for normal ribosome assembly and function in Escherichia coli. RNA. 2005 Jul;11(7):1141-52. doi: 10.1261/rna.2550105. Epub 2005 May 31. PMID:15928344 doi:http://dx.doi.org/10.1261/rna.2550105
- ↑ Phannachet K, Huang RH. Conformational change of pseudouridine 55 synthase upon its association with RNA substrate. Nucleic Acids Res. 2004 Feb 27;32(4):1422-9. Print 2004. PMID:14990747 doi:10.1093/nar/gkh287