DNA methyltransferase

Function DNA methyltransferase (DNMT) catalyzes the transfer of methyl group to DNA. Methylation is essential for gene expression. DNMT uses S-adenosyl methionine (SAM) as the methyl donor. The SAM analog S-adenosyl homocysteine (SAH) is a powerful inhibitor of DNMT. Methylguanine DNMT repairs the DNA lesion O6-methylguanine to guanine. Methylcytosine DNMT methylates the 5 position of cytosine. Methyladenine DNMT methylates the 6 position of adenine. Relevance DNMT inhibitors are being tested in cancer therapy. Disease The human ICF disorder is caused by mutations in DNMT 3B. Structural highlights The active site of methyladenine DNMT contains the methyl donor SAM.

spinqualitylabelsall models Adenine DNMT complex with SAM (PDB code 1nw5). Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D Structures of DNA methyltransferase3D Structures of DNA methyltransferase

Updated on 31-December-2015

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• Methylguanine DNMT
  • 1sfe – O6-methylguanine DNMT C terminal – Escherichia coli
  • 1qnt, 1eh6, 3llr - hO6-methylguanine DNMT – human
  • 1t38 - hO6-methylguanine DNMT (mutant) + DNA
  • 1t39, 1yfh - hO6-methylguanine DNMT + DNA
  • 1eh7 - hO6-methylguanine DNMT methylated
  • 1eh8 - hO6-methylguanine DNMT benzylated
    
  • 1mgt - O6-methylguanine DNMT – Thermococcus kodakarensis
    
  • 1wrj - O6-methylguanine DNMT - Sulfolobus tokodaii
  • 2g7h - O6-methylguanine DNMT – Methanocaldococcus jannaschii
  • 2kif, 2kim - O6-methylguanine DNMT – Vibrio parahaemolyticus - NMR
    
  • 4bhb, 4bhc - O6-methylguanine DNMT (mutant) – Mycobacterium tuberculosis
    
• Methylcytosine DNMT
  • 2qrv - h-cytosine DNMT
  • 3epz - h-cytosine DNMT replecation foci-targeting sequence
  • 3flg - h-cytosine DNMT ADD domain
  • 3a1a - h-cytosine DNMT PWWP domain
  • 1g55 - h-cytosine DNMT2 (mutant)
    
  • 3qkj - h-cytosine DNMT 3β
    
  • 3av4, 1khc - m-cytosine DNMT – mouse
  • 2uyc, 2uyh, 2uz4 - Hh-cytosine DNMT (mutant) + DNA + SAH
  • 3a1b - h-cytosine DNMT ADD domain + histone H3
  • 3pt6, 3pt9, 4da4 – m-cytosine DNMT + DNA

• • 3av5 - m-cytosine DNMT + SAH
  • 3av6 - m-cytosine DNMT + SAM
  • 3swr - h-cytosine DNMT + sinefungin
  • 1boo - cytosine DNMT (mutant) – Proteus vulgaris
    
  • 3qv2 - cytosine DNMT + SAH – Entamoeba histolytica
    
  • 2uyc, 2uyh, 2uz4, 1fjx – Hh-cytosine DNMT (mutant) + DNA + SAH - Haemophilus haemolyticus
    
  • 2c7o, 2c7p, 2c7q, 2c7r, 3pta – Hh-cytosine DNMT + DNA + S-adenonosyl cysteine
    
  • 1hmy – Hh-cytosine Haeiii DNMT + SAM
  • 1m0e - Hh-cytosine DNMT (mutant) + DNA + inhibitor
  • 2i9k - Hh-cytosine DNMT (mutant) + DNA
  • 2hr1 - Hh-cytosine DNMT + DNA + ADOHCY
  • 2z6q, 2z6u, 2zcj - Hh-cytosine DNMT (mutant) + DNA + ADOHCY
  • 1svu - Hh-cytosine DNMT (mutant)
    
  • 4fsx – c-cytosine DNMT + SAH – corn
    
  • 4ft2, 4ft4 – c-cytosine DNMT + histone H3 peptide
    
  • 4h0n - cytosine DNMT (mutant) – Spodoptera frugiperda
• Methyladenine DNMT
  • 1nw5 – RsN6-adenine DNMT + SAM – Rhodobacter sphaeroides
  • 1nw6 - RsN6-adenine DNMT + sinefungin
    
  • 1nw7 - RsN6-adenine DNMT + SAH
    
  • 1nw8 - RsN6-adenine DNMT (mutant)
  • 2ibs, 2ibt, 2ih2, 2ih4, 2ih5, 2np6, 2np7, 1g38 - Ta-adenine DNMT Taqi + DNA + cofactor analog – Thermus aquaticus
    
  • 1aqi – Ta-adenine DNMT Taqi + SAH
    
  • 1aqj – Ta-adenine DNMT Taqi + sinefugin
    
  • 2adm - Ta-adenine DNMT Taqi + SAM
    
  • 1g60 - adenine DNMT + SAM – Moraxella bovis