Adenosine dimethyltransferase

Function Adenosine dimethyltransferase (KsgA) dimethylates two adjacent adenines at the C-terminal of the 16S rRNA in the small 30S ribosomal subunit, thus modifying it. KsgA catalyzes the dimethylation of adenines 1518-1519 using S-adenosyl-methionine (SAM) as the methyl donor and producing S-adenosyl-homocysteine (SAH). Relevance KsgA confers resistance to the antibiotic kasugomycin. Structural highlights The active site pocket of KsgA in E, coli contains residues N113 and L114 which are part of motif IV SAM-dependent methyltrasferase sequence. The active site can acomodate both adenosine and methyladenosine.

spinqualitylabelsall models Structure of adenosine dimethyltransferase complex with RNA, SAH and K+ ion (purple) (PDB code 3ftf). Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D structures of adenosine dimethyltransferase3D structures of adenosine dimethyltransferase

Updated on 28-October-2015

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• Adenosine dimethyltransferase
  • 1qyr – EcKsgA – Escherichia coli
    
  • 3tpz – EcKsgA (mutant)
    
  • 3ftc, 3ftd – AaKsgA – Aquifex aeolicus
    
  • 3fut, 3fuv – TtKsgA – Thermus thermophilus
    
  • 3tqs – KsgA – Coxiella burnetii
    
  • 3uzu – KsgA – Burkholderia pseudomallei
    
  • 4jxj – KsgA – Rickettsia bellii
    
• Adenosine dimethyltransferase complex
  • 3fte – AaKsgA + RNA
    
  • 3ftf – AaKsgA + RNA + SAH
    
  • 3fuu, 3fuw, 3fux – TtKsgA + adenosine derivative
    
  • 3grr – MjKsgA (mutant) + SAH – Methanocaldococcus jannaschii
    
  • 3gru – MjKsgA (mutant) + AMP
    
  • 3grv – MjKsgA (mutant) + adenosine
    
  • 3gry – MjKsgA (mutant) + SAM
    
  • 3r9x – AaKsgA + GDPNP + RNA + GTPase
    
  • 4adv – EcKsgA + 30S ribosomal subunit – Cryo-EM