1qyr

2.1 Angstrom Crystal structure of KsgA: A Universally Conserved Adenosine Dimethyltransferase2.1 Angstrom Crystal structure of KsgA: A Universally Conserved Adenosine Dimethyltransferase

Structural highlights

1qyr is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.1Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RSMA_ECOLI Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits. Has also a DNA glycosylase/AP lyase activity that removes C mispaired with oxidized T from DNA, and may play a role in protection of DNA against oxidative stress.^[1] ^[2] ^[3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ van Buul CP, van Knippenberg PH. Nucleotide sequence of the ksgA gene of Escherichia coli: comparison of methyltransferases effecting dimethylation of adenosine in ribosomal RNA. Gene. 1985;38(1-3):65-72. PMID:3905517
↑ Zhang-Akiyama QM, Morinaga H, Kikuchi M, Yonekura S, Sugiyama H, Yamamoto K, Yonei S. KsgA, a 16S rRNA adenine methyltransferase, has a novel DNA glycosylase/AP lyase activity to prevent mutations in Escherichia coli. Nucleic Acids Res. 2009 Apr;37(7):2116-25. doi: 10.1093/nar/gkp057. Epub 2009 Feb, 17. PMID:19223326 doi:10.1093/nar/gkp057
↑ Connolly K, Rife JP, Culver G. Mechanistic insight into the ribosome biogenesis functions of the ancient protein KsgA. Mol Microbiol. 2008 Dec;70(5):1062-75. doi: 10.1111/j.1365-2958.2008.06485.x. PMID:18990185 doi:10.1111/j.1365-2958.2008.06485.x

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OCA

[1] van Buul CP, van Knippenberg PH. Nucleotide sequence of the ksgA gene of Escherichia coli: comparison of methyltransferases effecting dimethylation of adenosine in ribosomal RNA. Gene. 1985;38(1-3):65-72. PMID:3905517

[2] Zhang-Akiyama QM, Morinaga H, Kikuchi M, Yonekura S, Sugiyama H, Yamamoto K, Yonei S. KsgA, a 16S rRNA adenine methyltransferase, has a novel DNA glycosylase/AP lyase activity to prevent mutations in Escherichia coli. Nucleic Acids Res. 2009 Apr;37(7):2116-25. doi: 10.1093/nar/gkp057. Epub 2009 Feb, 17. PMID:19223326 doi:10.1093/nar/gkp057

[3] Connolly K, Rife JP, Culver G. Mechanistic insight into the ribosome biogenesis functions of the ancient protein KsgA. Mol Microbiol. 2008 Dec;70(5):1062-75. doi: 10.1111/j.1365-2958.2008.06485.x. PMID:18990185 doi:10.1111/j.1365-2958.2008.06485.x

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[2]

[3]

1qyr

2.1 Angstrom Crystal structure of KsgA: A Universally Conserved Adenosine Dimethyltransferase2.1 Angstrom Crystal structure of KsgA: A Universally Conserved Adenosine Dimethyltransferase

Structural highlights

Function

Evolutionary Conservation

See Also

References

Contents

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1qyr

2.1 Angstrom Crystal structure of KsgA: A Universally Conserved Adenosine Dimethyltransferase2.1 Angstrom Crystal structure of KsgA: A Universally Conserved Adenosine Dimethyltransferase

Structural highlights

Function

Evolutionary Conservation

See Also

References

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