3k1f

Crystal structure of RNA Polymerase II in complex with TFIIBCrystal structure of RNA Polymerase II in complex with TFIIB

Structural highlights 3k1f is a 13 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NonStd Res: Activity: DNA-directed RNA polymerase, with EC number 2.7.7.6 Resources: FirstGlance, OCA, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed To initiate gene transcription, RNA polymerase II (Pol II) requires the transcription factor IIB (B). Here we present the crystal structure of the complete Pol II-B complex at 4.3 A resolution, and complementary functional data. The results indicate the mechanism of transcription initiation, including the transition to RNA elongation. Promoter DNA is positioned over the Pol II active centre cleft with the 'B-core' domain that binds the wall at the end of the cleft. DNA is then opened with the help of the 'B-linker' that binds the Pol II rudder and clamp coiled-coil at the edge of the cleft. The DNA template strand slips into the cleft and is scanned for the transcription start site with the help of the 'B-reader' that approaches the active site. Synthesis of the RNA chain and rewinding of upstream DNA displace the B-reader and B-linker, respectively, to trigger B release and elongation complex formation. RNA polymerase II-TFIIB structure and mechanism of transcription initiation.,Kostrewa D, Zeller ME, Armache KJ, Seizl M, Leike K, Thomm M, Cramer P Nature. 2009 Nov 19;462(7271):323-30. PMID:19820686 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also RNA polymerase Transcription initiation factor

spinqualitylabelsall models 3k1f, resolution 4.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image