Vinculin

Vinculins (VCLs) are involved in adhesion by linking integrin molecules to the actin cytoskeleton. Its head domain (Vd1) can bind to talin or to alpha-actinin at their respective VCL Binding Sites (VBS). A splice variant of vinculin is meta-vinculin (m-VCL). The protein raver1 RNA Recognition Motif (RRM) forms a complex with VCL or m-VCL. The images at the left and at the right correspond to one representative Vinculin structure, i.e. crystal structure of human full-length Vinculin (1tr2). is achieved through a high affinity intramolecular interaction between tail (orange) and head (aqua) domains (1st6). Energetically, I997 is key to maintaining this autoinhibition. Metavinculin is a splice version of VCL containing an extra ca. 70 amino acids in the C-terminal domain.

3D Structures of Vinculin3D Structures of Vinculin

Updated on 28-September-2014

VinculinVinculin

3h2u – hVCL Vd1 + raver1 RRM - human
3h2v - hVCL C-terminal + raver1 RRM
2ibf, 2hsq, 2gww - hVCL Vd1 + SfVCL binding sites from Shigella flexneri
1tr2 – hVCL
1ydi - hVCL Vd1+hActinin VBS
1t01 - cVCL Vd1+mTalin VBS – chicken
1syq, 1rkc, 1rke – hVCL Vd1+hTalin VBS
1qkr – hVCL C-terminal
3zdl – cVCL Vd1 + amyloid β precursor protein N terminal

MetavinculinMetavinculin

3myi – m-VCL tail domain
3rf3 - hm-VCL + invasin IPAA
3s90 - hm-VCL head domain + mTalin-1 peptide
4dj9 - hm-VCL head domain + hTalin-1 peptide
3tj5 - hm-VCL head domain + Sca-family protein peptide
3tj6 - hm-VCL head domain + protein Ps 120 peptide
4ehp - hm-VCL head domain + catenin α-1 residues 277-382
3vf0 – hm-VCL residues 856-1134 + ribonucleoprotein PTB-binding
2gdc – cm-VCL Vd1+SfInvasin C-terminal
1xwj - cm-VCL Vd1+cTalin VBS3
1zvz, 1zw2, 1zw3, 1u6h - cm-VCL Vd1+cTalin rod
1st6 – cm-VCL
4e17, 4e18 - cm-VCL Vd1 + catenin α-1 VCL-binding domain

• Created with the participation of Susan Craig.