Sandbox Reserved 818

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This Sandbox is Reserved from 06/12/2018, through 30/06/2019 for use in the course "Structural Biology" taught by Bruno Kieffer at the University of Strasbourg, ESBS. This reservation includes Sandbox Reserved 1480 through Sandbox Reserved 1543.
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File:1prt.png

Template:STRUCTURE 1prt

PERTUSSIS TOXINPERTUSSIS TOXIN

About this StructureAbout this Structure

1prt is a 12 chain structure with sequence from Bordetella pertussis. The September 2005 RCSB PDB Molecule of the Month feature on Cholera Toxin by David S. Goodsell is 10.2210/rcsb_pdb/mom_2005_9. Full crystallographic information is available from OCA.


IntroductionIntroduction

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Binding of PTX to its cellular targetsBinding of PTX to its cellular targets

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Toxin entry and trafficking in target cellsToxin entry and trafficking in target cells

Mechanism of pertussis toxinMechanism of pertussis toxin

Pertussis toxin (PTX) acts on target cells through its A protomer which contains the enzymatically active S1 subunit.
This subunit catalyzes ADP-ribosylation of the α-subunit of trimeric G proteins, which disturbs functions of the target cells and therefore lead to various biological effects.

In facts, substrates of PTX are regulators of the membrane-bound adenylate cyclase. These G proteins bind GTP in order to transduce signals in the cell. When ADP-ribosylation by PTX occurs, the downregulation of the adenylate cyclase activity is inhibited.
This inhibition leads to increase cAMP levels in cells, which explains the amount of biological activities of the toxin.

-> picture of the active site of the S1 subunit
labeled in green : key residues surrounding the NAD-binding cavity (Arg9, Trp26, Cys41)
labeled in blue : catalytic residues (His35, Glu129)

PTX-catalysed ADP-ribosylation

Toxic effects of pertussis toxinToxic effects of pertussis toxin

See AlsoSee Also

ReferenceReference

[xtra 1]

  1. ↑ Stein PE, Boodhoo A, Armstrong GD, Cockle SA, Klein MH, Read RJ. The crystal structure of pertussis toxin. Structure. 1994 Jan 15;2(1):45-57. PMID:8075982

[xtra 1]

  1. ↑ Locht C, Coutte L, Mielcarek N. The ins and outs of pertussis toxin. FEBS J. 2011 Dec;278(23):4668-82. doi: 10.1111/j.1742-4658.2011.08237.x. Epub, 2011 Aug 4. PMID:21740523 doi:http://dx.doi.org/10.1111/j.1742-4658.2011.08237.x

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA, Lea Clusan, Paul Giroud
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