Vinculin
Vinculins (VCLs) are involved in adhesion by linking integrin molecules to the actin cytoskeleton. Its head domain (Vd1) can bind to talin or to alpha-actinin at their respective VCL Binding Sites (VBS). A splice variant of vinculin is meta-vinculin (m-VCL). The protein raver1 RNA Recognition Motif (RRM) forms a complex with VCL or m-VCL. The images at the left and at the right correspond to one representative Vinculin structure, i.e. crystal structure of human full-length Vinculin (1tr2). is achieved through a high affinity intramolecular interaction between tail (orange) and head (aqua) domains (1st6). Energetically, I997 is key to maintaining this autoinhibition.
3D Structures of Vinculin3D Structures of Vinculin
Update July 2012
3h2u – hVCL Vd1 + raver1 RRM - human
3h2v - hVCL C-terminal + raver1 RRM
2ibf, 2hsq, 2gww - hVCL Vd1 + SfVCL binding sites from Shigella flexneri
1tr2 – hVCL
1ydi - hVCL Vd1+hActinin VBS
1t01 - cVCL Vd1+mTalin VBS – chicken
1syq, 1rkc, 1rke – hVCL Vd1+hTalin VBS
1qkr – hVCL C-terminal
MetavinculinMetavinculin
3myi – m-VCL tail domain
3rf3 - hm-VCL + invasin IPAA
3s90 - hm-VCL head domain + mTalin-1 peptide
4dj9 - hm-VCL head domain + hTalin-1 peptide
3tj5 - hm-VCL head domain + Sca-family protein peptide
3tj6 - hm-VCL head domain + protein Ps 120 peptide
4ehp - hm-VCL head domain + catenin α-1 residues 277-382
2gdc – cm-VCL Vd1+SfInvasin C-terminal
1xwj - cm-VCL Vd1+cTalin VBS3
1zvz, 1zw2, 1zw3, 1u6h - cm-VCL Vd1+cTalin rod
1st6 – cm-VCL
4e17, 4e18 - cm-VCL Vd1 + catenin α-1 VCL-binding domain
- Created with the participation of Susan Craig.