Dethiobiotin synthetase

Dethiobiotin synthetase (DTBS) catalyzes the first ring closure in the biotin synthesis. The reaction transforms 7,8-diaminononanoate (DAN), ATP and CO2 to dethiobiotin (DTB), ADP and phosphate.

3D dethiobiotin synthetase3D dethiobiotin synthetase

1dbs, 1dts, 1byi – EcDTBS – Escherichia coli
2qmo – HpDTBS – Helicobacter pylori
3fgn – MtDTBS – Mycobacterium tuberculosis
3of5 – DTBS – Francicella tularensis

DTBS binary complex

3fmf – MtDTBS + DAN
1dad – EcDTBS + ADP
1dae - EcDTBS + nonanedioate derivative
3fmi – MtDTBS + aminopelargonic acid
1dai - EcDTBS + DAN

DTBS ternary complex

3qxc - HpDTBS + Mg + ATP
1daf - EcDTBS + DAN + ADP
1dag - EcDTBS + DAN + ATP derivative
3qxj - HpDTBS + Mg + ATP
3qxs - HpDTBS + Mg + phosphoaminophosphonic acid

DTBS quaternary complex

1dah - EcDTBS + DAN + Mn + ATP<BR / derivative
1bs1 - EcDTBS + DTB + Mg + ADP
1a82 - EcDTBS + DAN + Mg + ATP
3fpa - MtDTBS + DTB + Mg + phosphate
3mle - HpDTBS + Mg + phosphate + ADP
3qy0 - HpDTBS + Mg + phosphate + GDP

DTBS higher complexes

1dam - EcDTBS + DTB + Mg + phosphate + ADP
1dak - EcDTBS + DAN + mixed anhydride + Mg + phosphate + ADP
3qxh - HpDTBS + aminooctanoic acid + Mg + phosphate + ADP
3qxx - HpDTBS + aminooctanoic acid + Mg + phosphate + GTP