Pseudouridine synthase

Function tRNA pseudouridine synthase (PUS) catalyzes the conversion of tRNA uridine to tRNA pseudouridine. PUS are active in the ribosomal large subunit Ribosomal large subunit pseudouridine synthase and in the ribosomal small subunit Ribosomal small subunit pseudouridine synthase. Ribosomal large subunit pseudouridine synthase RluD is responsible for synthesis of pseudouridine from uracil in positions 1911, 1915 and 1917 in 23S ribosomal RNA. Probable tRNA pseudouridine synthase (PPUS) is a similar enzyme. Structural highlights The conversion of uridine to pseudouridine causes conformational change of PUS. (1ze2).

spinqualitylabelsall models tRNA pseudouridine synthase complex with RNA and pseudouridine monophosphate 1ze2 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D Structures of tRNA pseudouridine synthase3D Structures of tRNA pseudouridine synthase

Updated on 12-November-2018

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• tRNA pseudouridine synthase
  • 1dj0 – EcPUS I – Escherichia coli
    
  • 4iqm, 4its, 4j37, 4nz6 - hPUS Pus1 catalytic domain - human
    
  • 4nz7 - hPUS Pus1 catalytic domain (mutant)
    
  • 1vs3 – PUS TruA – Thermus thermophilus
    
  • 1sgv – PUS TruB – Mycobacterium tuberculosis
    
  • 1si7, 1szw, 1sb7 - EcPUS TruD
• tRNA pseudouridine synthase complex with RNA
  • 1k8w – EcTruB + stem-loop RNA
    
  • 1zl3 - EcTruB (mutant) + stem-loop RNA
    
  • 2nqp, 2nr0, 2nre – EcTruA + Leu-tRNA
    
  • 1ze2 - TmPUS B + RNA
    
  • 2ab4 - TmPUS B (mutant) + RNA
    
  • 1r3e, 1r3f - EcTruB + RNA
    
• Probable tRNA pseudouridine synthase
  • 3hjw, 3lwo, 3lwp, 3lwq, 3lwr, 3lwv, 3hax – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA – Pyrococcus furiosus
    
  • 3hay – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA + small nuclear RNP protein
    
  • 2hvy – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA + small nuclear RNP protein + small nuclear RNP Gar1-like protein
    
  • 3mqk - PfPUS Cbf5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA
    
  • 2rfk - PfPUS Cbf5 (mutant) + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA
    
  • 2ey4 - PfPUS Cbf5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein
    
  • 3hjy - PfPUS Cbf5 + ribosome biogenesis protein NOP10 + RNA
    
  • 2apo - PfPUS Cbf5 + ribosome biogenesis protein NOP10 - Methanocaldococcus jannaschii
    
• Ribosomal large subunit pseudouridine synthase
  • 2i82 - EcRluA catalytic domain + RNA
    
  • 4lab - EcRluB catalytic domain
    
  • 4lgt - EcRluB catalytic domain + RNA
    
  • 1xpi - EcRluC catalytic domain
    
  • 5vbb - hRluC-like
    
  • 1prz, 1qyu, 1v9f, 1v9k, 2ist - EcRluD catalytic domain
    
  • 2oml, 2olw - EcRluE catalytic domain
    
  • 2gml - EcRluF catalytic domain
    
  • 3dh3 - EcRluF catalytic domain + RNA
    
• Ribosomal small subunit pseudouridine synthase
  • 1vio - RsuA - Haemophilus influenzae
    
  • 1ksk - EcRsuA
    
  • 1ksl - EcRsuA + uracil
    
  • 1ksv - EcRsuA + UMP