7sol

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Crystal Structures of the bispecific ubiquitin/FAT10 activating enzyme, Uba6Crystal Structures of the bispecific ubiquitin/FAT10 activating enzyme, Uba6

Structural highlights

7sol is a 4 chain structure with sequence from Homo sapiens and Triticum aestivum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2500064Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

UBA6_HUMAN Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.[1] [2] [3]

See Also

References

  1. Zhu H, Zhou ZM, Huo R, Huang XY, Lu L, Lin M, Wang LR, Zhou YD, Li JM, Sha JH. Identification and characteristics of a novel E1 like gene nUBE1L in human testis. Acta Biochim Biophys Sin (Shanghai). 2004 Mar;36(3):227-34. doi:, 10.1093/abbs/36.3.227. PMID:15202508 doi:http://dx.doi.org/10.1093/abbs/36.3.227
  2. Jin J, Li X, Gygi SP, Harper JW. Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Nature. 2007 Jun 28;447(7148):1135-8. PMID:17597759 doi:http://dx.doi.org/nature05902
  3. Chiu YH, Sun Q, Chen ZJ. E1-L2 activates both ubiquitin and FAT10. Mol Cell. 2007 Sep 21;27(6):1014-23. PMID:17889673 doi:http://dx.doi.org/10.1016/j.molcel.2007.08.020

7sol, resolution 2.25Å

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