Sandbox UC 11
The structure of a complex containing the homeodomain repressor protein MATalpha2 and the MADS-box transcription factor MCM1 bound to DNA has been determined by X-ray crystallography at 2.25 A resolution.
Through the structure of this oligomer that was obtained by crystallography, we can see that HDAC9 is kept even after MEF2 binding to the chromosome. We can also easily observe a very well near the DNA binding site of MEF2.
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Directed evolution and Colicin7/Immunity-proteins complexes[1]Directed evolution and Colicin7/Immunity-proteins complexes[1]
of the immunity protein 9 (Im9, 1bxi, colored yellow), evolved variant R12-2 (lime), and immunity protein 7 (Im7, colored blue, 7cei) reveals their structural identity. However, when the immunity proteins-bound , they demonstrate somewhat different picture. <quiz display= simple> {¿Cuántas hélices alfa se encuentran en esta estructura?| Type= "[]"} - Ninguno + One - Cuatro </ Quiz> MCM1 bound to DNA [2] |
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- ↑ Levin KB, Dym O, Albeck S, Magdassi S, Keeble AH, Kleanthous C, Tawfik DS. Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Nat Struct Mol Biol. 2009 Oct;16(10):1049-55. Epub 2009 Sep 13. PMID:19749752 doi:10.1038/nsmb.1670
- ↑ MCM1 bound to DNA
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| 1mnm, resolution 2.25Å () | |||||||||
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| Resources: | FirstGlance, OCA, RCSB, PDBsum | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Additional LiteratureAdditional Literature
- ↑ Kleanthous C, Kuhlmann UC, Pommer AJ, Ferguson N, Radford SE, Moore GR, James R, Hemmings AM. Structural and mechanistic basis of immunity toward endonuclease colicins. Nat Struct Biol. 1999 Mar;6(3):243-52. PMID:10074943 doi:http://dx.doi.org/10.1038/6683
- ↑ Levin KB, Dym O, Albeck S, Magdassi S, Keeble AH, Kleanthous C, Tawfik DS. Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Nat Struct Mol Biol. 2009 Oct;16(10):1049-55. Epub 2009 Sep 13. PMID:19749752 doi:10.1038/nsmb.1670
External ResourcesExternal Resources
- Yahoo news coverage of luciferase studies funded research for military applications
- Luciferase at Wikipedia
- Luciferase: June 2006 Molecule of the Month as part of the series of tutorials that are at the RCSB Protein Data Bank and written by
- Lipase, the main article in Proteopedia.
- Molecular Playground/Pancreatic Lipase
- Lipase in Wikipedia