1dfe
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NMR STRUCTURE OF RIBOSOMAL PROTEIN L36 FROM THERMUS THERMOPHILUS
OverviewOverview
We have determined the solution NMR structure of the ribosomal protein L36, from Thermus thermophilus. L36 is the smallest protein in the large, subunit of the prokaryotic ribosome. The sequence contains three, completely conserved cysteine residues and one conserved histidine residue, in a C-X(2)-C-X(12)-C-X(4)-H motif. Extended X-ray absorption fine, structure spectroscopy was used to confirm that a purified L36 sample, contains an equimolar amount of zinc. The structure of L36 was determined, using simulated annealing based on NOE distance restraints, dihedral angle, restraints and hydrogen bond distance restraints derived from NMR spectra, of (15)N-labeled and non-labeled L36 samples at pH 7 and 12 degrees C, and, by imposing tetrahedral zinc ion coordination geometry. The L36 fold is, characterized by a triple-stranded antiparallel beta-sheet with the, zinc-binding site at one end. The structure of the zinc site is, well-determined and shows that the three cysteine sulphur atoms are, supported by hydrogen bonds to backbone amide protons. The conserved, histidine residue is located in a short 3(10)-helix and coordinates zinc, by the N(delta1) atom. The electrostatic surface potential and location of, conserved Arg, Lys and His side-chains suggest a large continuous L36-rRNA, interaction interface. The folding topology as well as position and, conformation of many conserved side-chains in L36 are very similar to, those of zinc-ribbon domains found in the archaeal transcription factor, TFIIB N terminus and the eukaryal transcription elongation factor hTFIIS C, terminus. Given the relative antiquity of the ribosome it is possible that, L36 reflects the parent of transcription-related zinc ribbons.
About this StructureAbout this Structure
1DFE is a Single protein structure of sequence from Thermus thermophilus with ZN as ligand. Full crystallographic information is available from OCA.
ReferenceReference
The solution structure of ribosomal protein L36 from Thermus thermophilus reveals a zinc-ribbon-like fold., Hard T, Rak A, Allard P, Kloo L, Garber M, J Mol Biol. 2000 Feb 11;296(1):169-80. PMID:10656825
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