1op8
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Crystal Structure of Human Granzyme A
OverviewOverview
Granzyme A (GzmA) belongs to a family of trypsin-like serine proteases, localized in cytoplasmic granules of activated lymphocytes and natural, killer (NK) cells. In contrast to the related granzyme B (GzmB), GzmA, forms a stable disulfide-linked homodimer and triggers target-cell death, in a caspase-independent way. Limited proteolysis of a high-molecular-mass, complex containing SET (also named putative HLA-associated protein II or, PHAPII), PHAPI (pp32, leucine-rich acidic nuclear protein) and HMG2 by, GzmA liberates NM23-H1, a Mg2+-dependent DNase that causes single-stranded, breaks in nuclear DNA. By analyzing the dimeric GzmA structure at a, resolution of 2.5 A, we determined the substrate-binding constraints and, selective advantages of the two domains arranged as a unique functional, tandem. The active sites of the two subunits point in opposite directions, and the nearby noncatalytic surfaces can function as exosites, presenting, substrates to the active site region of the adjacent partner in a manner, analogous to staphylokinase or streptokinase, which present plasminogen to, the cofactor-plasmin and cofactor-plasminogen complexes.
About this StructureAbout this Structure
1OP8 is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Granzyme A, with EC number 3.4.21.78 Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the apoptosis-inducing human granzyme A dimer., Hink-Schauer C, Estebanez-Perpina E, Kurschus FC, Bode W, Jenne DE, Nat Struct Biol. 2003 Jul;10(7):535-40. PMID:12819770
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