SelB: Difference between revisions
Eran Hodis (talk | contribs) No edit summary |
Eran Hodis (talk | contribs) No edit summary |
||
| Line 2: | Line 2: | ||
Elongation factor SelB uses several methods to recognize the selenocysteine insertion sequence found in messenger RNA. The insertion sequence forms a hairpin loop with an unstacked guanine base in the loop. This guanine forms | Elongation factor SelB uses several methods to recognize the selenocysteine insertion sequence found in messenger RNA. The insertion sequence forms a hairpin loop with an unstacked guanine base in the loop. This guanine forms | ||
<scene name='SelB_Recognition/1wsu-recognition1/ | <scene name='SelB_Recognition/1wsu-recognition1/3'>specific hydrogen bonds with the protein and with two bridging water molecules (shown in cyan in the jmol).</scene> | ||
The planar base is also sandwiched between | The planar base is also sandwiched between | ||
<scene name='Sandbox_14/1wsu-recognition2/2'>three amino acids</scene> in the protein, which form a tight hydrophobic pocket. This is best seen using a | <scene name='Sandbox_14/1wsu-recognition2/2'>three amino acids</scene> in the protein, which form a tight hydrophobic pocket. This is best seen using a | ||
<scene name='Sandbox_14/1wsu-recognition3/1'>spacefilling diagram.</scene> | <scene name='Sandbox_14/1wsu-recognition3/1'>spacefilling diagram.</scene> | ||
The overall shape of the hairpin is recognized by contacts with several | The overall shape of the hairpin is recognized by contacts with several | ||
<scene name=' | <scene name='SelB_Recognition/1wsu-recognition4/1'>arginine and lysine amino acids.</scene> | ||
In particular, notice the intimate contact formed by both the charged nitrogen and hydrophobic carbon chain of | In particular, notice the intimate contact formed by both the charged nitrogen and hydrophobic carbon chain of | ||
<scene name='Sandbox_14/1wsu-recognition5/1'>arginine 606.</scene> | <scene name='Sandbox_14/1wsu-recognition5/1'>arginine 606.</scene> | ||