1br5: Difference between revisions

Revision as of 19:34, 30 June 2008

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File:1br5.png

Template:STRUCTURE 1br5

RICIN A CHAIN (RECOMBINANT) COMPLEX WITH NEOPTERINRICIN A CHAIN (RECOMBINANT) COMPLEX WITH NEOPTERIN

Template:ABSTRACT PUBMED 9086280

About this StructureAbout this Structure

1BR5 is a Single protein structure of sequence from Ricinus communis. Full crystallographic information is available from OCA.

ReferenceReference

Structure-based identification of a ricin inhibitor., Yan X, Hollis T, Svinth M, Day P, Monzingo AF, Milne GW, Robertus JD, J Mol Biol. 1997 Mar 14;266(5):1043-9. PMID:9086280

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OCA

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-'''RICIN A CHAIN (RECOMBINANT) COMPLEX WITH NEOPTERIN'''
+===RICIN A CHAIN (RECOMBINANT) COMPLEX WITH NEOPTERIN===
-==Overview==
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-Ricin is a potent cytotoxin which has been used widely in the construction of therapeutic agents such as immunotoxins. Recently it has been used by governments and underground groups as a poison. There is interest in identifying and designing effective inhibitors of the ricin A chain (RTA). In this study computer-assisted searches indicated that pterins might bind in the RTA active site which normally recognizes a specific adenine base on rRNA. Kinetic assays showed that pteroic acid could inhibit RTA activity with an apparent Ki of 0.6 mM. A 2.3 A crystal structure of the complex revealed the mode of binding. The pterin ring displaces Tyr80 and binds in the adenine pocket making specific hydrogen bonds to active site residues. The benzoate moiety of pteroic acid binds on the opposite side of Tyr80 making van der Waals contact with the Tyr ring and forming a hydrogen bond with Asn78. Neopterin, a propane triol derivative of pterin, also binds to RTA as revealed by the X-ray structure of its complex with RTA. Neither pterin-6-carboxylic acid nor folic acid bind to the crystal or act as inhibitors. The models observed suggest alterations to the pterin moiety which may produce more potent and specific RTA inhibitors.
+The line below this paragraph, {{ABSTRACT_PUBMED_9086280}}, adds the Publication Abstract to the page
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+{{ABSTRACT_PUBMED_9086280}}
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 [[Category: Yan, X.]]
 [[Category: Glycosidase]]
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