2gwm: Difference between revisions

Revision as of 05:37, 4 May 2008

Crystal structure of the Salmonella SpvB ATR Domain

OverviewOverview

Salmonella spp. require the ADP-ribosyltransferase activity of the SpvB protein for intracellular growth and systemic virulence. SpvB covalently modifies actin, causing cytoskeletal disruption and apoptosis. We report here the crystal structure of the catalytic domain of SpvB, and we show by mass spectrometric analysis that SpvB modifies actin at Arg177, inhibiting its ATPase activity. We also describe two crystal structures of SpvB-modified, polymerization-deficient actin. These structures reveal that ADP-ribosylation does not lead to dramatic conformational changes in actin, suggesting a model in which this large family of toxins inhibits actin polymerization primarily through steric disruption of intrafilament contacts.

About this StructureAbout this Structure

2GWM is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.

ReferenceReference

A steric antagonism of actin polymerization by a salmonella virulence protein., Margarit SM, Davidson W, Frego L, Stebbins CE, Structure. 2006 Aug;14(8):1219-29. PMID:16905096 Page seeded by OCA on Sun May 4 05:37:42 2008

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OCA

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 [[Image:2gwm.gif|left|200px]]
- {{Structure
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-|GENE= mkaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium])
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-|RELATEDENTRY=[[2gwl|2GWL]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gwm OCA], [http://www.ebi.ac.uk/pdbsum/2gwm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gwm RCSB]</span>
-}}
 '''Crystal structure of the Salmonella SpvB ATR Domain'''
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 [[Category: Margarit, S M.]]
 [[Category: Stebbins, C E.]]
-[[Category: adp-ribosyltransferase]]
+[[Category: Adp-ribosyltransferase]]
-[[Category: salmonella]]
+[[Category: Salmonella]]
-[[Category: spvb]]
+[[Category: Spvb]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:37:42 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:22:37 2008''