1ya7: Difference between revisions

Revision as of 16:04, 3 May 2008

Implications for interactions of proteasome with PAN and PA700 from the 1.9 A structure of a proteasome-11S activator complex

OverviewOverview

Proteasomes are cylindrical structures that function in multiple cellular processes by degrading a wide variety of cytosolic and nuclear proteins. Substrate access and product release from the enclosed catalytic chamber occurs through axial pores that are opened by activator complexes. Here, we report high-resolution structures of wild-type and mutant archaeal proteasomes bound to the activator PA26. These structures support the proposal that an ordered open conformation is required for proteolysis and that its formation can be triggered by outward displacement of surrounding residues. The structures and associated biochemical assays reveal the mechanism of binding, which involves an interaction between the PA26 C terminus and a conserved lysine. Surprisingly, biochemical observations implicate an equivalent interaction for the unrelated ATP-dependent activators PAN and PA700.

About this StructureAbout this Structure

1YA7 is a Protein complex structure of sequences from Thermoplasma acidophilum and Trypanosoma brucei. Full crystallographic information is available from OCA.

ReferenceReference

The 1.9 A structure of a proteasome-11S activator complex and implications for proteasome-PAN/PA700 interactions., Forster A, Masters EI, Whitby FG, Robinson H, Hill CP, Mol Cell. 2005 May 27;18(5):589-99. PMID:15916965 Page seeded by OCA on Sat May 3 16:03:59 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1ya7.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1ya7 |SIZE=350|CAPTION= <scene name='initialview01'>1ya7</scene>, resolution 2.30&Aring;
+The line below this paragraph, containing "STRUCTURE_1ya7", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Proteasome_endopeptidase_complex Proteasome endopeptidase complex], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.25.1 3.4.25.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= psmA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2303 Thermoplasma acidophilum]), psmB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2303 Thermoplasma acidophilum])
+-->
-|DOMAIN=
+{{STRUCTURE_1ya7|  PDB=1ya7  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ya7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ya7 OCA], [http://www.ebi.ac.uk/pdbsum/1ya7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ya7 RCSB]</span>
-}}
 '''Implications for interactions of proteasome with PAN and PA700 from the 1.9 A structure of a proteasome-11S activator complex'''
@@ Line 32: / Line 29: @@
 [[Category: Robinson, H.]]
 [[Category: Whitby, F G.]]
-[[Category: archaeal proteasome]]
+[[Category: Archaeal proteasome]]
-[[Category: copmlex]]
+[[Category: Copmlex]]
-[[Category: open gate]]
+[[Category: Open gate]]
-[[Category: pa26]]
+[[Category: Pa26]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 16:03:59 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:00:47 2008''