8aey: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[8aey]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AEY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AEY FirstGlance]. <br>
<table><tr><td colspan='2'>[[8aey]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AEY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AEY FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8aey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8aey OCA], [https://pdbe.org/8aey PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8aey RCSB], [https://www.ebi.ac.uk/pdbsum/8aey PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8aey ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.05&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8aey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8aey OCA], [https://pdbe.org/8aey PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8aey RCSB], [https://www.ebi.ac.uk/pdbsum/8aey PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8aey ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/BFRB_MYCTU BFRB_MYCTU] Iron-storage protein that displays ferroxidase activity, catalyzing the oxidation of Fe(2+) ions into Fe(3+) ions, that can then be deposited as a ferric-oxide mineral core within the central cavity of the protein complex.<ref>PMID:21494619</ref>  
[https://www.uniprot.org/uniprot/BFRB_MYCTU BFRB_MYCTU] Iron-storage protein that displays ferroxidase activity, catalyzing the oxidation of Fe(2+) ions into Fe(3+) ions, that can then be deposited as a ferric-oxide mineral core within the central cavity of the protein complex.<ref>PMID:21494619</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The use of cryo-EM continues to expand worldwide and calls for good-quality standard proteins with simple protocols for their production. Here, a straightforward expression and purification protocol is presented that provides an apoferritin, bacterioferritin B (BfrB), from Mycobacterium tuberculosis with high yield and purity. A 2.12 A resolution cryo-EM structure of BfrB is reported, showing the typical cage-like oligomer constituting of 24 monomers related by 432 symmetry. However, it also contains a unique C-terminal extension (164-181), which loops into the cage region of the shell and provides extra stability to the protein. Part of this region was ambiguous in previous crystal structures but could be built within the cryo-EM map. These findings and this protocol could serve the growing cryo-EM community in characterizing and pushing the limits of their electron microscopes and workflows.
Mycobacterium tuberculosis ferritin: a suitable workhorse protein for cryo-EM development.,Gijsbers A, Zhang Y, Gao Y, Peters PJ, Ravelli RBG Acta Crystallogr D Struct Biol. 2021 Aug 1;77(Pt 8):1077-1083. doi:, 10.1107/S2059798321007233. Epub 2021 Jul 29. PMID:34342280<ref>PMID:34342280</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8aey" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==

Latest revision as of 09:45, 24 July 2024

3 A CRYO-EM STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS FERRITIN FROM TIMEPIX3 detector3 A CRYO-EM STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS FERRITIN FROM TIMEPIX3 detector

Structural highlights

8aey is a 24 chain structure with sequence from Mycobacterium tuberculosis H37Rv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 3.05Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BFRB_MYCTU Iron-storage protein that displays ferroxidase activity, catalyzing the oxidation of Fe(2+) ions into Fe(3+) ions, that can then be deposited as a ferric-oxide mineral core within the central cavity of the protein complex.[1]

See Also

References

  1. ↑ Khare G, Gupta V, Nangpal P, Gupta RK, Sauter NK, Tyagi AK. Ferritin Structure from Mycobacterium tuberculosis: Comparative Study with Homologues Identifies Extended C-Terminus Involved in Ferroxidase Activity. PLoS One. 2011 Apr 8;6(4):e18570. PMID:21494619 doi:10.1371/journal.pone.0018570

8aey, resolution 3.05Å

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