3qph: Difference between revisions

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<StructureSection load='3qph' size='340' side='right'caption='[[3qph]], [[Resolution|resolution]] 2.99&Aring;' scene=''>
<StructureSection load='3qph' size='340' side='right'caption='[[3qph]], [[Resolution|resolution]] 2.99&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3qph]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QPH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QPH FirstGlance]. <br>
<table><tr><td colspan='2'>[[3qph]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QPH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QPH FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.992&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF1743 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 ATCC 43587])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qph OCA], [https://pdbe.org/3qph PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qph RCSB], [https://www.ebi.ac.uk/pdbsum/3qph PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qph ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qph OCA], [https://pdbe.org/3qph PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qph RCSB], [https://www.ebi.ac.uk/pdbsum/3qph PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qph ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/TRMBR_PYRFU TRMBR_PYRFU] Inhibits transcription of the trehalose/maltose transport gene cluster (malE operon) and of the maltodextrin transport gene cluster (mdxE operon). Acts by binding to two different operator sequences in both promoters, preventing polymerase recruitment and transcription.<ref>PMID:12426307</ref> <ref>PMID:16135241</ref>  
TrmB is a repressor that binds maltose, maltotriose, and sucrose, as well as other alpha-glucosides. It recognizes two different operator sequences controlling the TM (Trehalose/Maltose) and the MD (Maltodextrin) operon encoding the respective ABC transporters and sugar-degrading enzymes. Binding of maltose to TrmB abrogates repression of the TM operon but maintains the repression of the MD operon. On the other hand, binding of sucrose abrogates repression of the MD operon but maintains repression of the TM operon. The three-dimensional structure of TrmB in complex with sucrose was solved and refined to a resolution of 3.0 A. The structure shows the N-terminal DNA binding domain containing a winged-helix-turn-helix (wHTH) domain followed by an amphipathic helix with a coiled-coil motif. The latter promotes dimerization and places the symmetry mates of the putative recognition helix in the wHTH motif about 30 A apart suggesting a canonical binding to two successive major grooves of duplex palindromic DNA. This suggests that the structure resembles the conformation of TrmB recognizing the pseudopalindromic TM promoter but not the conformation recognizing the nonpalindromic MD promoter.
 
The three-dimensional structure of TrmB, a transcriptional regulator of dual function in the hyperthermophilic archaeon Pyrococcus furiosus in complex with sucrose.,Krug M, Lee SJ, Boos W, Diederichs K, Welte W Protein Sci. 2013 Jun;22(6):800-8. doi: 10.1002/pro.2263. Epub 2013 May 8. PMID:23576322<ref>PMID:23576322</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3qph" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc 43587]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Boos, W]]
[[Category: Pyrococcus furiosus]]
[[Category: Diederichs, K]]
[[Category: Boos W]]
[[Category: Krug, M]]
[[Category: Diederichs K]]
[[Category: Lee, S J]]
[[Category: Krug M]]
[[Category: Welte, W]]
[[Category: Lee S-J]]
[[Category: Transcription]]
[[Category: Welte W]]
[[Category: Transcriptional regulator]]

Latest revision as of 13:48, 21 February 2024

The three-dimensional structure of TrmB, a global transcriptional regulator of the hyperthermophilic archaeon Pyrococcus furiosus in complex with sucroseThe three-dimensional structure of TrmB, a global transcriptional regulator of the hyperthermophilic archaeon Pyrococcus furiosus in complex with sucrose

Structural highlights

3qph is a 1 chain structure with sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.992Å
Ligands:, , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TRMBR_PYRFU Inhibits transcription of the trehalose/maltose transport gene cluster (malE operon) and of the maltodextrin transport gene cluster (mdxE operon). Acts by binding to two different operator sequences in both promoters, preventing polymerase recruitment and transcription.[1] [2]

See Also

References

  1. ↑ Lee SJ, Engelmann A, Horlacher R, Qu Q, Vierke G, Hebbeln C, Thomm M, Boos W. TrmB, a sugar-specific transcriptional regulator of the trehalose/maltose ABC transporter from the hyperthermophilic archaeon Thermococcus litoralis. J Biol Chem. 2003 Jan 10;278(2):983-90. PMID:12426307 doi:10.1074/jbc.M210236200
  2. ↑ Lee SJ, Moulakakis C, Koning SM, Hausner W, Thomm M, Boos W. TrmB, a sugar sensing regulator of ABC transporter genes in Pyrococcus furiosus exhibits dual promoter specificity and is controlled by different inducers. Mol Microbiol. 2005 Sep;57(6):1797-807. PMID:16135241 doi:10.1111/j.1365-2958.2005.04804.x

3qph, resolution 2.99Å

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