7pcx: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| Line 3: | Line 3: | ||
<StructureSection load='7pcx' size='340' side='right'caption='[[7pcx]], [[Resolution|resolution]] 1.40Å' scene=''> | <StructureSection load='7pcx' size='340' side='right'caption='[[7pcx]], [[Resolution|resolution]] 1.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7pcx]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PCX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PCX FirstGlance]. <br> | <table><tr><td colspan='2'>[[7pcx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_sp. Rhodococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PCX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PCX FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OEH:[9-[2-carboxy-5-[2-[2-(6-chloranylhexoxy)ethoxy]ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethyl-azanium'>OEH</scene | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OEH:[9-[2-carboxy-5-[2-[2-(6-chloranylhexoxy)ethoxy]ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethyl-azanium'>OEH</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pcx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pcx OCA], [https://pdbe.org/7pcx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pcx RCSB], [https://www.ebi.ac.uk/pdbsum/7pcx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pcx ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pcx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pcx OCA], [https://pdbe.org/7pcx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pcx RCSB], [https://www.ebi.ac.uk/pdbsum/7pcx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pcx ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/DHAA_RHOSO DHAA_RHOSO] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 18: | Line 19: | ||
</div> | </div> | ||
<div class="pdbe-citations 7pcx" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 7pcx" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Dehalogenase 3D structures|Dehalogenase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Frei | [[Category: Rhodococcus sp]] | ||
[[Category: Hiblot | [[Category: Frei M]] | ||
[[Category: Johnsson | [[Category: Hiblot J]] | ||
[[Category: Tarnawski | [[Category: Johnsson K]] | ||
[[Category: Tarnawski M]] | |||
Latest revision as of 16:04, 1 February 2024
X-RAY STRUCTURE OF THE HALOALKANE DEHALOGENASE HALOTAG7-Q165W LABELED WITH A CHLOROALKANE-TETRAMETHYLRHODAMINE FLUOROPHORE SUBSTRATEX-RAY STRUCTURE OF THE HALOALKANE DEHALOGENASE HALOTAG7-Q165W LABELED WITH A CHLOROALKANE-TETRAMETHYLRHODAMINE FLUOROPHORE SUBSTRATE
Structural highlights
FunctionPublication Abstract from PubMedSelf-labeling protein tags such as HaloTag are powerful tools that can label fusion proteins with synthetic fluorophores for use in fluorescence microscopy. Here we introduce HaloTag variants with either increased or decreased brightness and fluorescence lifetime compared with HaloTag7 when labeled with rhodamines. Combining these HaloTag variants enabled live-cell fluorescence lifetime multiplexing of three cellular targets in one spectral channel using a single fluorophore and the generation of a fluorescence lifetime-based biosensor. Additionally, the brightest HaloTag variant showed up to 40% higher brightness in live-cell imaging applications. Engineered HaloTag variants for fluorescence lifetime multiplexing.,Frei MS, Tarnawski M, Roberti MJ, Koch B, Hiblot J, Johnsson K Nat Methods. 2022 Jan;19(1):65-70. doi: 10.1038/s41592-021-01341-x. Epub 2021 Dec, 16. PMID:34916672[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||