6spp: Difference between revisions
No edit summary |
No edit summary |
||
| Line 3: | Line 3: | ||
<StructureSection load='6spp' size='340' side='right'caption='[[6spp]], [[Resolution|resolution]] 1.49Å' scene=''> | <StructureSection load='6spp' size='340' side='right'caption='[[6spp]], [[Resolution|resolution]] 1.49Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6spp]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[6spp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SPP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SPP FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.49Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id=' | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6spp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6spp OCA], [https://pdbe.org/6spp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6spp RCSB], [https://www.ebi.ac.uk/pdbsum/6spp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6spp ProSAT]</span></td></tr> | ||
< | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/SYM_ECOLI SYM_ECOLI] Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.[HAMAP-Rule:MF_00098] | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 21: | Line 19: | ||
</div> | </div> | ||
<div class="pdbe-citations 6spp" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 6spp" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Escherichia coli]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Mechulam Y]] | |||
[[Category: Mechulam | [[Category: Nigro G]] | ||
[[Category: Nigro | [[Category: Schmitt E]] | ||
[[Category: Schmitt | |||
Latest revision as of 15:45, 24 January 2024
Structure of the Escherichia coli methionyl-tRNA synthetase variant VI298Structure of the Escherichia coli methionyl-tRNA synthetase variant VI298
Structural highlights
FunctionSYM_ECOLI Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.[HAMAP-Rule:MF_00098] Publication Abstract from PubMedPolypeptides containing beta-amino acids are attractive tools for the design of novel proteins having unique properties of medical or industrial interest. Incorporation of beta-amino acids in vivo requires the development of efficient aminoacyl-tRNA synthetases specific of these non-canonical amino acids. Here, we have performed a detailed structural and biochemical study of the recognition and use of beta(3)-Met by Escherichia coli methionyl-tRNA synthetase (MetRS). We show that MetRS binds beta(3)-Met with a 24-fold lower affinity but catalyzes the esterification of the non-canonical amino acid onto tRNA with a rate lowered by three orders of magnitude. Accurate measurements of the catalytic parameters required careful consideration of the presence of contaminating alpha-Met in beta(3)-Met commercial samples. The 1.45 A crystal structure of the MetRS: beta(3)-Met complex shows that beta(3)-Met binds the enzyme essentially like alpha-Met, but the carboxylate moiety is mobile and not adequately positioned to react with ATP for aminoacyl adenylate formation. This study provides structural and biochemical bases for engineering MetRS with improved beta(3)-Met aminoacylation capabilities. Use of beta(3)-methionine as an amino acid substrate of Escherichia coli methionyl-tRNA synthetase.,Nigro G, Bourcier S, Lazennec-Schurdevin C, Schmitt E, Marliere P, Mechulam Y J Struct Biol. 2019 Dec 17:107435. doi: 10.1016/j.jsb.2019.107435. PMID:31862305[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||